Background: Using Brucella abortus Strain 19 (S19) to control bovine brucellosis is restricted due to induce antibodies to the O-side chain of the smooth lipopolysaccharide (LPS) which may be difficult to differentiate vaccinated and infected animals. Furthermore, it is virulent for humans and can induce abortion to cattle. Objectives: The aim of...

background:   h2-eb1 molecule which is the homolog of human hla-drb1 is proposed to be associated with allergic rhinitis (ar). construction of   h2-eb1 knockout animal models provides a tool to elucidate the role of h2-eb1 and ar pathogenesis. objective:   to establish the h2-eb1 knockout model and investigate the h2-eb1 functions in   h2-eb1 knockout mice as a model of ar. methods: the cre/lox...

One way to get an insight into the potential interaction between a pair of genes is to compare the phenotype of the double knockout organism to the phenotype we would expect if the genes were independent. However, since gene knockouts are expensive and time-consuming, we would like to be able to predict gene interactions. We used a dataset consisting of 352 yeast genes for which only a few doub...

Background:   H2-EB1 molecule which is the homolog of Human HLA-DRB1 is proposed to be associated with allergic rhinitis (AR). Construction of   H2-Eb1 knockout animal models provides a tool to elucidate the role of H2-EB1 and AR pathogenesis. Objective:   To establish the H2-Eb1 knockout model and investigate the H2-EB1 functions in   H2-Eb1 knockout mice as a model of AR. Methods: The Cre/Lox...

Regulatory On-Off Minimization (ROOM) is a model for predicting the behavior of metabolic networks in response to gene knockouts. It is based on minimizing the number of significant flux changes (hence, on/off) with respect to the wild-type. ROOM outperforms a previously suggested model, Minimization Of Metabolic Adjustment (MOMA), whose minimization metric is Euclidian. Furthermore, ROOM shows...

In the two following studies from the same group, the authors used an interesting and sophisticated design of genetically modified mice that express diphtheria toxin upon Cre recombination, the expression of which itself is driven by the endogenous regulatory sequences of a target gene. This strategy thereby results in specific ablation of an entire cellular population, like kisspeptin or KISS1...

Quantitative high-throughput screenings (qHTSs) for genotoxicity are conducted as part of comprehensive toxicology screening projects. The most widely used method is to compare the dose-response data of a wild-type and DNA repair gene knockout mutants, using model-fitting to the Hill equation (HE). However, this method performs poorly when the observed viability does not fit the equation well, ...