background: after radiation therapy (rt), some health hazards including dna damages may occur where melatonin can play a protective role due to free radical generation. on the other hand, serious accidental overexposures may occur during rt due to nuclear accidents which necessitate the need for study on exposure to high-dose radiations during treatments. objective: the aim of this study was to...

conclusions the obtained results indicate that rsm is an effective method for the optimization of culture conditions for hes gene expression in e. coli. results a maximum rhes mrna level of 376.16% was obtained under the following conditions: 0.6 mm iptg, 7 hours post-induction time, and 0.9 cell density before induction. the level of rhes mrna was significantly correlated with post-induction t...

the gene encoding the human basic fibroblast growth factor (hbfgf) has been already chemically-synthesized and cloned in pet-3a expression vector (pasteur institute of iran). in the present study, we compared the level of expression of this synthetic hbfgf and its related cdna in escherichia coli. the pbr322-cdna of hbfgf supplied by dr. seno (from molecular biology dept, okaido prefectural uni...

The gene encoding the human basic fibroblast growth factor (hbFGF) has been already chemically-synthesized and cloned in pET-3a expression vector (Pasteur Institute of Iran). In the present study, we compared the level of expression of this synthetic hbFGF and its related cDNA in Escherichia coli. The pBR322-cDNA of hbFGF supplied by Dr. Seno (from Molecular Biology Dept, Okaido prefectural uni...

plasma membrane h+-atpase is a major integral membrane protein with a role in various physiological processes including abiotic stress response. to study the effect of nacl on the expression pattern of a gene encoding the plasma membrane h+-atpase, an experiment was carried out in a completely random design with three replications. a pair of specific primers was designed based on the sequence o...

The present study was conducted to investigate the expression level of cytochrome P450 enzyme in detoxification of water soluble fractions (WSFs) of crude oil in Persian sturgeon, Acipenser persicus. Fish were exposed to WSFs at concentrations of 0 (control), 10, 20, and 50% of the LC50 for 14 days, and then, the expression level of P450 gene was assayed in gill and liver samples. According to ...