A total of 10–20% plasma membrane proteins are anchored by glycosylphosphatidylinositol (GPI). GPI is attached to transamidase (GPI‐T), which contains five subunits named PIGK, PIGS, PIGT, PIGU, and GPAA1. We previously reported that PIGT localizes near the nucleus in Drosophila . However, localizations other four remain unknown. Here, we show a catalytic subunit GPI‐T, mainly endoplasmic retic...

Over 100 glycosylphosphatidylinositol-anchored proteins (GPI-APs) are encoded in the mammalian genome. It is not well understood how these targeted and translocated to endoplasmic reticulum (ER). Here, we reveal that many GPI-APs, such as CD59, CD55, CD109, utilize human SND2 (hSND2)-dependent ER targeting machinery. We also found signal recognition particle receptors seem cooperate with hSND2 ...

The transferrin receptor of Trypanosoma brucei (TbTfR) is a heterodimer glycosylphosphatidylinositol (GPI)-anchored ESAG6 subunit and an ESAG7 subunit. To investigate whether the GPI-anchor essential for function TbTfR, with transmembrane domain instead (ESAG6tmd) was inducibly expressed in bloodstream form trypanosomes. It shown that ESAG6tmd able to dimerise TbTfR can bind transferrin. Fracti...

Paroxysmal nocturnal hemoglobinuria (PNH) is a hematopoietic stem cell disorder in which clonal cells defective in glycosylphosphatidylinositol (GPI) biosynthesis are expanded, leading to complement-mediated hemolysis. PNH is often associated with bone marrow suppressive conditions, such as aplastic anemia. One hypothetical mechanism for the clonal expansion of GPI(-) cells in PNH is that the m...

This note reports partial results related to the Gaussian product inequality (GPI) conjecture for joint distribution of traces Wishart matrices. In particular, several GPI-related from Wei [32] and Liu et al. [15] are extended in two ways: by replacing power functions with more general classes functions, usual multivariate gamma distributional assumptions trace-Wishart assumption. These finding...

The general features of the glycosylphosphatidylinositol (GPI) signal have been conserved in evolution. To test whether the requirements for GPI attachment are indeed the same in mammalian cells and parasitic protozoa, we expressed the prototype GPI-linked protein of Trypanosoma brucei, the variant surface glycoprotein (VSG), in COS cells. Although large amounts of VSG were produced, only a sma...

The proportions of glucose phosphate isomerase (GPI-1) allozymes produced by early (Gpi-1sa/Gpi-1sb female X Gpi-1sc/Gpi-1sc male)F1 mouse embryos were analysed by quantitative cellulose acetate electrophoresis. Technical controls showed that this system is extremely sensitive, quantitatively reproducible and quite accurate. Genetic controls established that the Gpi-1sa/Gpi-1sb mothers were hom...

Basic fibroblast growth factor (bFGF) is a hematopoietic cytokine that stimulates stromal and stem cell growth. It binds to a glycosylphosphatidylinositol (GPI)-anchored heparan sulfate proteoglycan on human bone marrow (BM) stromal cells. The bFGF-proteoglycan complex is biologically active and is released by addition of exogenous phosphatidylinositol-specific phospholipase C. In this study, w...