Vacuoles isolated by a mechanical slicing method from developing tubers of Jerusalem artichoke (Helianthus tuberosus L.) contain activities of the two principal enzymes responsible for fructan synthesis: sucrose-sucrose fructosyl transferase and fructan-fructan fructosyl transferase. Both enzymes are associated with the vacuolar sap and not with the tonoplast. In vacuoles isolated from dormant ...

Differences have been shown between ryegrass and fescue within the Festulolium subline introgression family for fructan synthesis, metabolism, and polymer-size traits. It is well-established that there is considerable variation for water-soluble carbohydrate and fructan content within perennial ryegrass. However there is much still to be discovered about the fructan polymer pool in this species...

A total of 720 1-d-old male Ross broilers (BW of 48.0±0.3 g) were used to evaluate the effects of dietary levan fructan supplementation on growth performance, meat quality, relative organ weight, cecal microflora, and excreta noxious gas emission in broilers. This experiment lasted 31 d. Broilers were randomly allotted to 1 of 3 dietary treatments: 1) CON, basal diet, 2) CON+0.25% fructan (FC1)...

This study was conducted to determine the effect of dietary fructan supplementation on growth performance, nutrient digestibility, meat quality, fecal microbial flora, and fecal noxious gas emission in finishing pigs. A total of 96 finishing pigs [(Yorkshire×Landrace)×Duroc] with an average BW of 73.1±2.5 kg were used in a 6-wk study. Pigs were randomly allotted to 1 of 3 dietary treatments: 1)...

(Fructan production in Vernonia herbacea (Vell.) Rusby is related to adequate nitrogen supply and period of cultivation). Previous studies showed that plants of Vernonia herbacea grown for one year under a limited nitrogen supply presented reduced growth and higher fructan content than plants treated with sufficient nitrogen supply. However, the total fructan production was similar in both plan...

In chicory taproot, the inulin-type fructans serve as carbohydrate reserve. Inulin metabolism is mediated by fructan active enzymes (FAZYs): sucrose:sucrose 1-fructosyltransferase (1-SST; fructan synthesis), fructan:fructan-1-fructosyltransferase (1-FFT; fructan synthesis and degradation), and fructan 1-exohydrolases (1-FEH1/2a/2b; fructan degradation). In developing taproot, fructan synthesis ...

Administering high concentrations of fructan to horses has resulted in laminitis. Cool season grasses accumulate fructan, which is estimated as the difference between water soluble carbohydrates (WSC; sucrose, fructose, glucose, fructans) and ethanol soluble carbohydrates (ESC; sucrose, fructose, glucose). Reducing fructan content in hay is critical for susceptible horses. The objective was to ...

Water-soluble carbohydrate composition of mature (ceased expanding) leaf blades and the elongation zone of developing leaf blades was characterized in wheat (Triticum aestivum L.), tall fescue (Festuca arundinacea Schreb.), and timothy (Phleum pratense L.). These species were chosen because they differ in mean degree of polymerization (DP) of fructan in the mature leaf blade. Our objective was ...

Graminan-type fructans are temporarily stored in wheat (Triticum aestivum) stems. Two phases can be distinguished: a phase of fructan biosynthesis (green stems) followed by a breakdown phase (stems turning yellow). So far, no plant fructan exohydrolase enzymes have been cloned from a monocotyledonous species. Here, we report on the cloning, purification, and characterization of two fructan 1-ex...

Most plants store starch or Suc as reserve carbohydrates, but about 15% of all flowering plant species store fructans, which are linear and branched polymers of Fru. Among the plants that store fructans are many of significant economic importance, such as cereals (e.g. barley, wheat, and oat), vegetables (e.g. chicory, onion, and lettuce), ornamentals (e.g. dahlia and tulip), and forage grasses...