Production of high quality embryos in vitro needs an efficient in vitro fertilization (IVF). Seminal origin is one of the important factors that affects the success of in vitro embryo production. So our goal was to determine the effect of using fresh and frozen semen in fertilization on developmental competence and cryo-survival of buffalo embryos. Buffalo oocytes wer...

semen from six adult male black bengal goats (capra hircus) was collected to compare the fresh and frozen-thawed semen quality and investigate its relationship with the fertility. each collected samples was divided into two parts: one part of semen was used as fresh and another part was filled into 0.50 ml straws, sealed, cooled (5 ˚c) and equilibrated for freezing.in both cases, semen was dilu...

Semen from six adult male Black Bengal goats (Capra hircus) was collected to compare the fresh and frozen-thawed semen quality and investigate its relationship with the fertility. Each collected samples was divided into two parts: One part of semen was used as fresh and another part was filled into 0.50 ml straws, sealed, cooled (5 ˚C) and equilibrated for freezing.In both cases, semen was dilu...

this study was undertaken to compare the efficiency of fresh and frozen-thawed buck semen on in vitro fertilization (ivf) of goat oocytes. cumulus oocytes complexes (cocs) were collected by aspiration of 2-6 mm diameter follicles, which were obtained from slaughterhouse. upon grading, only normal quality cocs were maturated in tcm-199 for 48 hours. the percentage of cocs reached to the m-ii sta...

This study was undertaken to compare the efficiency of fresh and frozen-thawed buck semen on in vitro fertilization (IVF) of goat oocytes. Cumulus oocytes complexes (COCs) were collected by aspiration of 2-6 mm diameter follicles, which were obtained from slaughterhouse. Upon grading, only normal quality COCs were maturated in TCM-199 for 48 hours. The percentage of COCs reached to the M-II sta...

This study was undertaken to compare the efficiency of fresh and frozen-thawed buck semen on in vitro fertilization (IVF) of goat oocytes. Cumulus oocytes complexes (COCs) were collected by aspiration of 2-6 mm diameter follicles, which were obtained from slaughterhouse. Upon grading, only normal quality COCs were maturated in TCM-199 for 48 hours. The percentage of COCs reached to the M-II sta...

Farrowing rate and number of pigs born alive were compared after use of either frozen semen or combined fresh and frozen semen matings in nulliparous gilts. Estrus was synchronized using Altrenogest (AT: Regu-Mate® Solution, Hoescht Roussel Vet, Warren, New Jersey) and detected with a mature boar. The farrowing rate was 74% for females inseminated with frozen semen, and 88% for females insemina...

In the first experiment, osmotic pressure of semen and seminal plasma in a semen sample from each of the 20 mature Nili-Ravi buffalo bulls was determined. In the second experiment, effects of osmotic pressure on motility (%), plasma membrane integrity (%) and viability (%) in fresh and frozen-thawed semen samples from each of the seven mature Nili-Ravi buffalo bulls was determined. In the first...

Bangladesh possesses a tropical goat breed popularly known as Black Bengal goat. They are dwarf in size and noted to be famous for their adaptability, fertility, prolificacy, delicacy of meat and superior skin quality (Husain et al. 1996). It is well adapted to hot and humid climate and usually produces twins and triplets. However, it has been observed that there exists sizeable genetic variati...

Semen quality and its relationship to fertility are major concern in animal production. The aim of this study was to assess the relationship between frozen thawed sperm characteristics and fertility following in vivo fertility through Artificial Insemination(AI). Semen samples were collected from four buffalo bulls. Semen volume and sperm concentration appeared to be significantly different (P0...