Atthough it is generally believed that under natural conditionso freeze-tolerant organisms can survive only if ice formation is restricted to the extracellular space, in 1959 R. W. Salt reported that fat body cefls of the freeze-tolerant gall fly, Eurosta solidaginis (Diptera: Tephritidae)' survive intracellular freezing. Using cryomicroscopy, intratellular freezing was observed at -4.6*0.1oC f...

ELISPOT assays are routinely used to measure immune responses of T cells in fresh and frozen splenocytes preparations; however, standardized methods for murine ELISPOT are not widely available. Freezing cells can significantly impact the function of T cells. The aim of this study was to optimize cryopreservation protocols to retain antigen-specific T cell function at similar levels as freshly i...

Research up to 1993 is reviewed. Various methods for processing, freezing and thawing of semen have been elaborated and their effect on survival and fertility of spermatozoa have been examined in vitro or in fertility tests. Diluents initially used for freezing bull semen had their limitations and were modified or replaced by new media. The freezing diluents investigated included citrate-sugar-...

Background Cryopreservation procedure for poultry semen is not reliable due to structural and biochemical damages which can lead to reduction in sperm quality and fertility. Development of cryopreservation medium has crucial effects for successful recovery of sperm after freezing. The objective of this study was assessment of cryopreservation of rooster semen using hand-made cryopreservation me...

Problem statement: Boar sperm cells are sensitive to the freezing process, which compromises viability of frozen-thawed sperm. In a constant search for minimizing or suppressing sperm cell damage caused by the temperature and osmolarity changes during the freezing process, crioprotective and antioxidant substances have been added to the freezing media, such as coconut water, in order to increas...

Freezing at -80 degrees C, lyophilization, preservation in distilled water, and storage in different culture media were performed in order to find a suitable method that allowed a prolonged storage of Malassezia spp. Freezing at -80 degrees C was the only method successful at maintaining all species.

The paper deals with cryopreservation of bifidobacteria and bacteriophages. Effect of high rates of cooling on survival ability and physiological activity of bifidobacteria is evaluated. The possibility to use rapid freezing for cryopreservation of stock Pseudomonas phage cultures is examined. The study indicates that rapid cooling kinetics appeared to lead to minimal losses in viability and ac...

Background: ROS production is a well-established phenomenon during sperm cryopreservation. Therefore, supplementation of freezing media with antioxidant is an inevitable event. The aim of this study was to assess whether a cell permeable superoxide dismutase agent such as MnTE, can further improve the quality of frozen/ thawed semen sample using a commercially optimized sperm cryopreservation m...

the effects of sucrose and some regulators on freezing tolerance in chickpea studied. four weeks in vitro explants were cultured in three different ms media without growth regulators and with normal level of sucrose (30 gl-1) (a), without growth regulators and with reduced sucrose (10 gl-1) (b) and medium supplemented with lmg gl-1 bap and 0.1 mgi1 iba and with 30 gi-1sucrose (c) for two weeks....