a study in egypt during june 2012-august 2013 revealed that there were a disastrous out break of foot and mouth disease where sat2 strain have been identified as the main reason for its  occurrence. trained and untrained persons routinely perform uncontrolled treatments leading to sever economic losses. the present paper represents an intervention that was developed to empower the community to ...

background and aims: the aim of this study was application of gamma irradiated fmdv antigen for liquid phase blocking sandwich elisa (b-elisa) to quantify guinea pig anti fmdv type a87/irn antibody. methods: fmdv type a87/irn was propagated to a titer of 107.5/ml tcid50 and then irradiated by gamma irradiator. the irradiated 146 s antigen was purified by sucrose gradient centrifugation after in...

foot and mouth disease (fmd) is the most contagious disease of cloven-hoofed animals, causingsignificant economical losses in livestock animals. in this study fmd virus type a87/irn was cultured andmultiplied on bhk21 cells. the fmd virus was titrated by the tissue culture infection dose50 method(tcid50 /ml), the virus titration was 107.5.. the fmd virus samples were irradiated and inactivated ...

objective: the aim of this study was to construct a pcdna3.1+ vector containing fmdv type o/irn/1/2007-vp1 gene, protein expression in bhkt7 cells and evaluation of immune response in balb/c mice. materials and methods: fmdv type o/irn/1/2007 was isolated from a cattle in ray in 2007 and serotyped. the purified vp1 gene was sub-cloned into the ptz57r/t vector and pcdna3.1+ expression vector. t...

foot and mouth disease (fmd) is a highly contagious viral disease of cloven-hooved livestock and wildlife results to relatively high mortality in young animals. despite the numerous reports of fmd-related death in neonates, there is little data available on various aspects of fmd in lambs. this report describes myocarditis associated with fmd in five, one week to three months old lambs. the lam...

during 2010-2011, real-time pcr procedure was used to detecting fmdv rna on 147 epithelium samples from the field. in this survey, for real-time pcr from 3d gene segment as conserve region selected for tracking all of seven serotypes fmdv. the assay detected the viral rna in all serotypes of fmdv. the rrt-pcr specifically detected fmd virus in sample with greater sensitivity than our convention...

background and aims: accurate and rapid diagnosis is necessary for effective control and prevention of foot-and-mouth disease (fmd). in present study, was evaluated real time reverse transcription-polymerase chain reaction (rrt-pcr) assay along with diagnostic routine methods for the detection of all seven serotypes of fmd virus (fmdv), namely o, c, a, sat1, 2, 3 and asia 1 in biological sample...

results the pellet of the 146s antigen of fmd virus serotype a was developed at a concentration of sucrose 50%. absorbance rate of the foot-and-mouth disease virus serotypes a at wavelengths of 240, 259 and 280 nm was 1.238, 1.573 and 1.157, respectively. moreover, the amount of 146s antigen at the same wavelengths was 163.416, 207.636 and 152.724 μg/ml, respectively. the amount of purified pro...

introduction: foot-and-mouth disease (fmd) is a highly contagious and economically devastating viral disease of livestock that is categorized in list a of animal diseases by the world organisation for animal health (oie). vaccination is effective against fmd and the vaccine production centers largely use the industrial ultra-filtration and chromatography in order to remove the cellular proteins...

foot and mouth disease (fmd) is highly contagious disease among cloven-hoofed animals. fmd virus has structural and non-structural proteins. vp1 is the most immunogenic structural peptides of fmd virus, applied for major vaccine studies. objective: construction of pet28-vp1 cassette for fmd virus type o/irn/2010 and expression vp1 peptide as the most immunogenic antigen was the aim of this stud...