We describe a "fluorescence protection immunoassay," in which formation of an immune complex of a fluorescer-labeled antigen sterically protects the fluorescer from binding by antibodies to it. Competitive binding of unlabeled antigen by its antibody prevents formation of the fluorescer-labeled antigen immune complex, and allows anti-fluorescein to quench the fluorescence by binding to the fluo...

Fluorescence excitation transfer immunoassay is a form of homogeneous assay suitable for the measurement of both large and small molecules. F6rster in 1948 [1] demonstrated that energy can be transferred through space from a fluorescent donor to an acceptor molecule; immunoassays based on this observation have now been developed. In fluorescence excitation transfer immunoassay, antibody is labe...

peroxyoxalate system includes an oxalate ester, peroxide and a fluorescer, the energy resulted from the reaction of oxalate ester and peroxide is transferred to fluorescer and cause it to fluorsc. the effect of reactants chemical structure, their concentration, solvent media (polarity and viscosity) and the temperature, on the intensity of the illuminated light with respect to time have been st...

Peroxyoxalate system includes an oxalate ester, peroxide and a fluorescer, The energy resulted from the reaction of oxalate ester and peroxide is transferred to fluorescer and cause it to fluorsc. The effect of reactants chemical structure, their concentration, solvent media (polarity and viscosity) and the temperature, on the intensity of the illuminated light with respect to time have bee...

the reaction of hydrogen peroxide and  pheny -9-acridinecarboxylate in the presence of rhodamin b as a fluorophore leads to the emission of either bright blue light  or bright red light, depending on the structure of fluorescer and reaction conditions. the light emitter responsible for the chemi luminescence (cl) reaction is acridone and that the blue light produced with out the fluorescers is ...

The reaction of hydrogen peroxide and  pheny -9-acridinecarboxylate in the presence of rhodamin B as a fluorophore leads to the emission of either bright blue light  or bright red light, depending on the structure of fluorescer and reaction conditions. The light emitter responsible for the Chemi Luminescence (CL) reaction is acridone and that the blue light produced with out the fluorescers...

The chemiluminescence (CL) arising from the reaction of bis(2,4,6-trichlorophenyl)oxalate (TCPO) with H2O2 in the presence of a novel blue fluorescer, furandicarboxylate derivative, has been studied. The relationship between the CL intensity and concentrations of peroxyoxalate, sodium salicylate, H2O2 and furandicarboxylate derivative as fluorescer has been investigated. Kinetic parameters for ...

the chemiluminescence (cl) arising from the reaction of bis(2,4,6-trichlorophenyl)oxalate (tcpo) with h2o2 in the presence of a novel blue fluorescer, furandicarboxylate derivative, has been studied. the relationship between the cl intensity and concentrations of peroxyoxalate, sodium salicylate, h2o2 and furandicarboxylate derivative as fluorescer has been investigated. kinetic parameters for ...

In systems containing singlet-oxygen and aromatic fluorescers energy transfer from singletoxygen dimers to the dye should be observable by emission of the fluorescer. In order to prove this hypothesis, externally generated singlet-oxygen (M g) was bubbled through the solutions of dyes (chlorophyll a, eosin y, rhodamine b, luminol, rubrene and acridine orange) in organic solvents. Luminescence c...