introduction non-invasive fluorescent reflectance imaging (fri) is used for accessing physiological and molecular processes in biological media. the aim of this article is to separate the overlapping emission spectra of quantum dots within tissue-equivalent phantom using svd, jacobi svd, and nmf methods in the fri mode. materials and methods in this article, a tissue-like phantom and an optical...

Introduction Non-invasive Fluorescent Reflectance Imaging (FRI) is used for accessing physiological and molecular processes in biological media. The aim of this article is to separate the overlapping emission spectra of quantum dots within tissue-equivalent phantom using SVD, Jacobi SVD, and NMF methods in the FRI mode. Materials and Methods In this article, a tissue-like phantom and an optical...

Reporter gene-based strategies are widely used in experimental oncology. Bioluminescence imaging (BLI) using the firefly luciferase (Fluc) as a reporter gene and d-luciferin as a substrate is currently the most widely employed technique. The present paper compares the performances of BLI imaging with fluorescence imaging using the near infrared fluorescent protein (iRFP) to monitor brain tumor ...

We report a novel approach to quantitatively assess gene transfection efficacy using dual-modality microendoscopy that can simultaneously monitor both laser scanning reflectance and fluorescence imaging. The system uses a 500-μm-diameter coherent fiber bundle and permits 3.5-μm lateral resolution. Both reflectance and fluorescence images obtained from two silicon avalanche photodetectors are di...

We present three-dimensional fluorescence yield tomography of a tissue phantom in a noncontact reflectance imaging setup. The method employs planar illumination with modulated light and frequency domain fluorescence measurements made on the illumination plane. An adaptive finite-element algorithm is used to handle the ill-posed and computationally demanding inverse image reconstruction problem....

Near-infrared fluorescence-enhanced diffuse optical tomography (fDOT) is used to localize tumors in mice using fluorescent nanoparticles as a blood pool contrast agent. The infrared dye DiR is loaded in the lipid core of nontargeted nanoparticles (DiR-lipidots) and injected systemically via the tail vein in mice bearing U87 tumors. Distribution and time-course of DiR-lipidots are followed using...

We present a spectral model for predicting the fluorescent emission and the total reflectance of color halftones printed on optically brightened paper. By relying on extended Neugebauer models, the proposed model accounts for the attenuation by the ink halftones of both the incident exciting light in the UV wavelength range and the emerging fluorescent emission in the visible wavelength range. ...

Hyperspectral imaging is an emerging technology in the field of biomedical engineering which may be used as a non-invasive modality for cancer characterization. In this project, we propose to investigate near-infrared (NIR) hyperspectral imaging for the characterization of canine mammary cancer. Nearinfrared hyperspectral imaging has been used for the detection of various kinds of human cancer;...

Hyperspectral imaging(HI) is an emerging technology in the field of biomedical engineering which may be used as a non-invasive modality for cancer characterization. In this project, we propose to investigate hyperspectral imaging for the characterization of gastric cancer. The hyperspectral imaging has been used for the detection of various kinds of human cancer; breast, gastric, prostate and t...

I propose a model for predicting the total reflectance of color halftones printed on paper incorporating fluorescent brighteners. The total reflectance is modeled as the additive superposition of the relative fluorescent emission and the pure reflectance of the color print. The fluorescent emission prediction model accounts for both the attenuation of light by the halftone within the excitation...