Background: Recent improvement in treatments of cancer has significantly increased survival rates of cancer patients. Unfortunately these treatments such as chemotherapy and radiotherapy can sever damage to the ovaries. Ovarian cryopreservation prior to chemo/ radiotherapy and auto-transplantation post-treatment can restore fertility to cancer patients. Hypoxia in the transplant from delayed re...

The application of biomaterials used in regenerative endodontics should be traceable. In this study, we checked some basic effects of rendering a fibrin hydrogel radiopaque using an iodine-based contrast agent (iodixanol) approved for systemic application. Fibrin hydrogels were prepared from a fibrin sealant (Tisseel) using either an isotonic iodixanol solution (Visipaque 320, test) or Tris buf...

BACKGROUND Polyethylene glycol (PEG) hydrogel is a biocompatible semi-adherent gel like substance that can potentially augment nerve repair much like a fibrin sealant. Potential advantages of this substance include fast preparation and set up time, as well as adhesion inhibiting properties. The purpose of this study was to perform an initial evaluation of PEG hydrogel in this application. MET...

EVALUATION OF FIBRIN HYDROGEL SCAFFOLD EFFECT ON OSTEOGENIC DIFFERENTIATION WHARTONS’S JELLY DERIVED MESENCHYMAL STEM CELLS

BACKGROUND Osteoarthritis (OA) is a degenerative joint disease affecting approximately 27 million Americans, and even more worldwide. OA is characterized by degeneration of subchondral bone and articular cartilage. In this study, a chondrogenic fibrin/hyaluronic acid (HA)-based hydrogel seeded with bone marrow-derived mesenchymal stem cells (BMSCs) was investigated as a method of regenerating t...

BACKGROUND Stringent control of proteolytic activity represents a major therapeutic approach for wound-bed preparation. OBJECTIVES We tested whether a protease-modulating polyacrylate- (PA-) containing hydrogel resulted in a more efficient wound-bed preparation of venous leg ulcers when compared to an amorphous hydrogel without known protease-modulating properties. METHODS Patients were ran...

The hydrogel synthesized from biocompatible polyaspartamide, and oxidized dextran had stronger adhesion properties when tested on porcine skin than the commercially available fibrin glue.

Cell-matrix adhesion is one of the important interactions that regulates stem cell survival, self-renewal, and differentiation. Our previous report (Wu SC, Chang JK, Wang CK, Wang GJ, Ho ML. Biomaterials 31: 631-640, 2010) indicated that a microenvironment enriched with hyaluronan (HA) initiated and enhanced chondrogenesis in human adipose-derived stem cells (hADSCs). We further hypothesize tha...

OBJECTIVES To design an in-situ gelling hydrogel capable of solidifying rapidly under physiologic conditions into a hydrogel capable of adhering tissue surfaces together. DESIGN Multifunctional polymers containing covalently bound peptide substrates of transglutaminase were designed. EXPERIMENTAL VARIABLE Enzyme cross-linked hydrogels were compared with commercial fibrin tissue adhesive. ...

A variety of skin substitutes that restore epidermal and dermal structures are currently available on the market. However, the main focus in research and clinical application lies on dermal and epidermal substitutes whereas the development of a subcutaneous replacement (hypodermis) is often disregarded. In this study we used fibrin sealant as hydrogel scaffold to generate a three-layered skin s...