A marked enhancement in the production of cell-bound phytase in Pichia anomala was achieved in cyclic fed batch fermentation (47333 UL -1 ) in comparison with that in batch (11569 UL -1 ) and fixed volume fed batch (36911 UL -1 ) fermentations in cane molasses medium. Phytase productivity was sustainable in cyclic fed batch fermentation over seven days, and therefore, this mode of fed batch fer...

modeling of fermentation processes is so complicated and uncertain; therefore it is necessary to provide a robust and appropriate dynamic optimization method. in order to obtain the maximum amount of yeast (saccharomyces cerevisiae), the bioreactor must be operated under optimal conditions. to determine substrate feeding in a fed-batch bioreactor, a simulated annealing (sa) approach was examine...

Astaxanthin is a main carotenoid pigment. Fed-batch fermentation bioprocesses of astaxanthin in 2.0 M fermentor have been investigated. Moreover, pH shift control was employed in fed-batch fermentation at 2.0 M scale. The results of astaxanthin fermentation showed that the fed-batch fermentation with pH shift strategy was the best among all the experiments, and the maximum astaxanthin concentra...

Recombinant alkaline polygalacturonate lyase (PGL) production by recombinant Pichia pastoris GS115 was selected as a model to study as a continuous culture strategy for enhancing heterologous protein production based on controlling methanol feeding (CCCM culture) or on dual carbon source feeding (CCCD culture). Using the CCCM process with a dry cell weight of 75 g/L regulated by controlling met...

To intensify the brewing process and increase the fermenter productivity, many breweries throughout the world have been embracing high-gravity brewing. However, increasing original wort concentration can cause a negative effect on yeast fermentation performance. This study focused on the application of fed-batch fermentation in high-gravity brewing. High gravity worts of 24oBx (using 30% high m...

The principal objective of this study was to evaluate the kinetics of dihydroxyacetone production by Gluconobacter frateurii CGMCC 5397 under different oxygen volumetric mass transfer coefficient (kLa) conditions in submerged bioreactors using biodiesel-derived crude glycerol as the carbon source. kLa is a key fermentation parameter for the production of dihydroxyacetone. Cultivations were cond...

The production of lipids by oleaginous yeast and fungi becomes more important because these lipids can be used for biodiesel production. To understand the process of lipid production better, we developed a model for growth, lipid production and lipid turnover in submerged batch fermentation. This model describes three subsequent phases: exponential growth when both a C-source and an N-source ar...

Alkali-pretreated sugarcane bagasse fiber was subjected to fed-batch simultaneous saccharification and fermentation (SSF) with a prehydrolysis process to increase the solids loading and produce a high concentration of ethanol. The hydrolysis medium and yeast feeding modes were investigated to determine suitable conditions for high sugar yield and ethanol production. Batch addition resulted in a...

In this paper optimal control algorithms for two E. coli fed-batch fermentations are developed. Fed-batch fermentation processes of E. coli strain MC4110 and E. coli strain BL21(DE3)pPhyt109 are considered. Simple material balance models are used to describe the E. coli fermentation processes. The optimal feed rate control of a primary metabolite process is studied and a biomass production is u...

By applying a fed-batch strategy, production of Penicillium occitanis mannanases could be almost doubled as compared to a batch cultivation on acacia seeds (76 versus 41 U/mL). Also, a 10-fold increase of enzyme activities was observed from shake flask fermentation to the fed-batch fermentation. These production levels were 3-fold higher than those obtained on coconut meal. The high mannanase p...