excretory/secretory antigen was prepared by culturing live adult worms of haemonchus contortus in rpmi 1640 medium at a concentration of 50 worms per ml in a culture-flask at 37 ˚c for 24 hr and the culture supernatant was used as antigen. the e/s antigen was purified by thiol-sepharose affinity chromatography. on western blot analysis, it was demonstrated that thiol-purified antigen showed a s...

background: we investigated the variations of heamathologic and biochemical parameters with different parasitic burdens and the correlation between fecal egg counts and fluke number in livers of sheep naturally infected with dicrocoelium dendriticum . methods: in order to detect excretion of d. dendriticum eggs, fecal samples from 120 sheep of different age groups were collected individually at...

OBJECTIVE To determine effects of pituitary pars intermedia dysfunction (Cushing's disease) and age on fecal egg count and time to egg reappearance after anthelmintic treatment in horses residing in similar environments. DESIGN Cross-sectional study. ANIMALS 29 healthy horses (4 to 35 years old) and 13 horses with PPID (13 to 33 years old). PROCEDURES Fecal egg counts were performed by us...

To establish a reliable diagnostic measure for equine Anoplocephala perfoliata infection, the impact of deworming was examined in 12 Thoroughbreds to which bithionol (5-10 mg/kg body weight) was administered and feces were examined by the modified Wisconsin method using sucrose solution. One day after the administration, cestode eggs were detected in previously fecal egg-negative 3 horses and i...

Goat production is widespread in the tropics. Goats are very susceptible to gastrointestinal nematode infection, but there is less evidence of their genetic resistance. Genetic resistance of Creole goats to gastrointestinal nematodes has been studied at Guadeloupe in the French West Indies since 1995. The objective of this research was to investigate genetic variation for resistance to gastroin...

BACKGROUND Examination of bile could be useful to diagnose Platynosomum spp.-induced cholangitis in cats. Obtaining bile via percutaneous ultrasound-guided cholecystocentesis (PUC) is possible but raises safety concerns in cats with severe cholecystitis. OBJECTIVES The objectives of this study were to investigate the use of PUC to collect bile samples from cats with known platynosomosis and t...

Abstract This study was performed to assess the possible relationship between gastrointestinal strongylid infections and milk quantity quality in donkeys used for production. Individual fecal samples were collected from 16 multiparous lactating jennies of Amiatina breed at their 6th month lactation. Statistical analysis carried out eggs per gram faeces (EPG) parameters. All animals found shed c...

Day-to-day fluctuations of both circulating anodic antigen (CAA) and circulating cathodic antigen (CCA) in serum and urine were examined simultaneously in a group of Schistosoma mansoni-infected individuals from Burundi and compared with each other and with fecal egg count fluctuations. Significant correlations were found between fecal egg counts and circulating antigens (CAA and CCA) and betwe...

Aim: The present study was aimed to determine the resistance against albendazole, fenbendazole, levamisole and closantel in gastrointestinal (GI) nematodes of sheep. Introduction: Anthelmintics are used traditionally as an integral part of helminthic control strategies for grazing livestock to prevent production losses from parasitic infections. The continuous and indiscriminate use of the same...

This study was conducted on four sheep breeds, Karakul, Kajli, Thalli and Kachhi for their natural resistance against Haemonchus contortus. After deworming, 13 animals of each breed were ingested with doses of 5000 L3 of Haemonchus contortus and then evaluation of each was made on the basis of fecal egg count (FEC), body weight, FAMACHA score and packed cell volume (PCV) at different days of in...