نتایج جستجو برای: FBPA

تعداد نتایج: 87  

Journal: :Journal of applied microbiology 2010
D Muñoz-Provencio G Pérez-Martínez V Monedero

AIMS To characterize the functionality of the Lactobacillus casei BL23 fbpA gene encoding a putative fibronectin-binding protein. METHODS AND RESULTS Adhesion tests showed that L. casei BL23 binds immobilized and soluble fibronectin in a protease-sensitive manner. A mutant with inactivated fbpA showed a decrease in binding to immobilized fibronectin and a strong reduction in the surface hydro...

Journal: :FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2002
Frank Rose Friedrich Grimminger Jutta Appel Mathias Heller Volker Pies Norbert Weissmann Ludger Fink Sebastian Schmidt Stefanie Krick Gieri Camenisch Max Gassmann Werner Seeger Jörg Hänze

Chronic lung hypoxia causes vascular remodeling with pulmonary artery smooth muscle cell (SMCPA) hyperplasia, resulting in pulmonary hypertension and cor pulmonale. We investigated SMCPA and pulmonary artery adventitial fibroblasts (FBPA) for their proliferative response to hypoxia. Strong SMCPA growth occurred under hypoxic conditions in SMCPA/FBPA co-cultures, but not in SMCPA monocultures. S...

Journal: :The Journal of biological chemistry 2007
Andrey Galkin Liudmila Kulakova Eugene Melamud Ling Li Chun Wu Patrick Mariano Debra Dunaway-Mariano Theodore E Nash Osnat Herzberg

Class I and class II fructose-1,6-bisphosphate aldolases (FBPA), glycolytic pathway enzymes, exhibit no amino acid sequence homology and utilize two different catalytic mechanisms. The mammalian class I FBPA employs a Schiff base mechanism, whereas the human parasitic protozoan Giardia lamblia class II FBPA is a zinc-dependent enzyme. In this study, we have explored the potential exploitation o...

Journal: :Biochemical Society transactions 2004
E Lorentzen B Siebers R Hensel E Pohl

FBPA (fructose-1,6-bisphosphate aldolase) catalyses the reversible aldol condensation of glyceraldehyde 3-phosphate and dihydroxyacetone phosphate to form fructose 1,6-bisphosphate. Two classes of FBPA, which rely on different reaction mechanisms, have so far been discovered, class I mainly found in Eucarya and class II mainly in Bacteria. Only recently were genes encoding proteins with FBPA ac...

2016
David R. Hall Charles S. Bond Gordon A. Leonard C. Ian Watt Alan Berry William N. Hunter

ion of a proton from DHAP C1 would be feasible. Glu of FBPA corresponds to Glu in TBPA, which is also positioned on a glycine-rich loop. Due to the lack of reliable electron density, this residue has not been included in the TBPA model and it is presumed to be flexible and disordered. The sequence conservation of this loop in TBPA and FBPA is high (Fig. 2), which implies that Glu could adopt a ...

Journal: :Journal of nuclear medicine : official publication, Society of Nuclear Medicine 2004
Hsin-Ell Wang Ai-Ho Liao Win-Ping Deng Pi-Fang Chang Jyh-Cheng Chen Fu-Du Chen Ren-Shen Liu Jin-Shin Lee Jeng-Jong Hwang

UNLABELLED L-p-Boronophenylalanine (BPA) has been applied as a potential boron carrier for the treatment of malignant glioma in clinical boron neutron capture therapy (BNCT) since 1994. To provide the pharmacokinetics of BPA for clinical use of BNCT in Taiwan, 4-borono-2-(18)F-fluoro-L-phenylalanine-fructose ((18)F-FBPA-Fr) was synthesized and the biologic characteristics of this radiotracer in...

2014
Kohei Hanaoka Tadashi Watabe Sadahiro Naka Yasukazu Kanai Hayato Ikeda Genki Horitsugi Hiroki Kato Kayako Isohashi Eku Shimosegawa Jun Hatazawa

BACKGROUND Boron neutron capture therapy (BNCT) is a molecular radiation treatment based on the (10)B (n, α) (7)Li nuclear reaction in cancer cells, in which delivery of (10)B by 4-borono-phenylalanine conjugated with fructose (BPA-fr) to the cancer cells is of critical importance. The PET tracer 4-borono-2-(18) F-fluoro-phenylalanine (FBPA) has been used to predict the accumulation of BPA-fr b...

Objective(s): L-4-borono-2-18F-fluoro-phenylalanine (L-[18F]FBPA), a substrate of L-type amino acid transporter 1 (LAT1), is a tumor-specific probe used in positron emission tomography (PET). On the other hand, it has not been examined whether another isomer D-[18F]FBPA accumulates specifically in the tumor. He...

2011
Anna Gardberg Jan Abendroth Janhavi Bhandari Banumathi Sankaran Bart Staker

Fructose bisphosphate aldolase (FBPA) enzymes have been found in a broad range of eukaryotic and prokaryotic organisms. FBPA catalyses the cleavage of fructose 1,6-bisphosphate into glyceraldehyde 3-phosphate and dihydroxyacetone phosphate. The SSGCID has reported several FBPA structures from pathogenic sources, including the bacterium Brucella melitensis and the protozoan Babesia bovis. Bioinf...

Journal: :The Biochemical journal 2007
Ali G Khan Stephen R Shouldice Leslie W Tari Anthony B Schryvers

The acquisition of iron from transferrin by Gram-negative bacterial pathogens is dependent on a periplasmic ferric-ion-binding protein, FbpA. FbpA shuttles iron from the outer membrane to an inner membrane transport complex. A bound phosphate anion completes the iron co-ordination shell of FbpA and kinetic studies demonstrate that the anion plays a critical role in iron binding and release in v...

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