Exoglucanase was produced using coffee husk, the by-product of coffee processing industries, as the sole carbon source using economical fermentation strategies like solid-state fermentation (SSF). The effects of process parameters such as pH, moisture and fermentation duration on exoglucanase synthesis was investigated using response surface methodology (RSM) based on three factors and five lev...

Chaetomium erraticum was capable of producing all the three components of a cellulase enzyme system including exoglucanase, endoglucanases, and β-glucosidase extracellularly. However, the cultivation conditions and the medium composition markedly affected the ability of microorganism to synthesize various enzymes. Exoglucanase was highest under static conditions, while endoglucanase and β-gluco...

Abstract Glucanases provide an excellent option for pre-treatment of cellulose and improvement in properties microfibrillated cellulose. In our research, we have used two different enzyme formulations, a purified β-1,4-endoglucanase, β-1,4-exoglucanase rich mixture the focus study is on effect high protein applications PFI refined-microfibrillated (2,500 rev). At applications, fibers showed imp...

Cellulolytic enzymes are generally composed of multienzyme systems and are divided into three classes, endoglucanase, exoglucanase and β-glucosidase. They work synergistically to hydrolyze cellulose molecules to glucose. Fungal exoglucanase (CBH) is a unique enzyme, capable of degrading highly ordered crystalline cellulose. Here we report the isolation of both genomic and cDNA clone and coding ...

Cellulose is a heterogeneous polysaccharide, and its enzymatic hydrolysis requires endoglucanase, exoglucanase (cellobiohydrolase), and beta-glucosidase to work together. We summarize the most commonly used assays for individual enzymes and cellulase mixture.

The purpose of this study was to isolate cellulolytic enzyme producing bacteria which may able to utilize banana pseudostem as sole carbon source for production of cellulolytic enzymes. This was attempted by isolating 10 bacteria from banana pseudostem waste. Out of the screened, isolate NAULH-5 showed high cellulolytic activity. The isolate NAULH-5 produced highest 5.97 U/mg endoglucanase, 2.2...

The activity of components of the extracellular cellulase system of the thermophilic fungus Sporotrichum thermophile showed appreciable differences between strains; beta-glucosidase (EC 3.2.1.21) was the most variable component. Although its endoglucanase (EC 3.2.1.4) and exoglucanase (EC 3.2.1.91) activities were markedly lower, S. thermophile degraded cellulose faster than Trichoderma reesei....

The alkaline-tolerant marine-derived fungus Chaetomium globosum was tested for the production of enhanced levels of cellulases and free phenolics under highly alkaline conditions using agro wastes (cotton seed, sugar cane bagasse) as substrates under solid state fermentation (SSF) processes. In both the agro wastes used, an increase in cellulases (β-endoglucanase, β-Glucosidase, and β-exoglucan...

six strain of trichoderma (t. viride, t. harzianum, t. reesei, t. longibrachiatum, t. koningii, t. virens) were isolated and used for extracellular enzyme production. species of trichoderma are collected from the surrounding soil of suger beet root fields in east iran, and held in vitro cultured pda. tcm and tfm are used for the growth of spores and induceing the production of cellulase. finall...