this study was designed to compare the effectiveness of different activation treatments for activation of in vitro matured oocytes and their developmental potency in mcr2aa medium so as to obtain maximum number of embryos. a total of 1090 cumulus oocyte complexes (coc’s) were collected from 480 ovaries. in vitro matured oocytes were randomly divided into four groups. group 1 in vitro matured oo...

This study was designed to compare the effectiveness of different activation treatments for activation of in vitro matured oocytes and their developmental potency in mCR2aa medium so as to obtain maximum number of embryos. A total of 1090 cumulus oocyte complexes (COC’s) were collected from 480 ovaries. In vitro matured oocytes were randomly divided into four groups. Group 1 in vitro matured oo...

the rate constants and activation parameters are reported for the oxidation of methanol, ethanol, propanol, and isopropanol by 2,2’-bipyridyl chromium peroxide (bpcp) over the temperature range of 35° to 50°c.

Mechanisms of alcoholic pancreatitis remain unknown. Previously, we showed that ethanol feeding sensitizes rats to pancreatitis caused by CCK-8, at least in part, by augmenting activation of the proinflammatory transcription factor NF-kappaB. To elucidate the mechanism of sensitization, here we investigate the effect of ethanol on Ca(2+)- and PKC-mediated pathways of CCK-induced NF-kappaB activ...

The rate constants and activation parameters are reported for the oxidation of methanol, ethanol, propanol, and isopropanol by 2,2’-bipyridyl chromium peroxide (BPCP) over the temperature range of 35° to 50°C.

Gukovskaya, Anna S., Saeed Hosseini, Akihiko Satoh, Jason H. Cheng, Kyung J. Nam, Ilya Gukovsky, and Stephen J. Pandol. Ethanol differentially regulates NFB activation in pancreatic acinar cells through calcium and protein kinase C pathways. Am J Physiol Gastrointest Liver Physiol 286: G204–G213, 2004. First published September 4, 2003; 10.1152/ajpgi.00088.2003.—Mechanisms of alcoholic pancreat...

To understand the mechanisms by which ethanol inhibits hepatocyte proliferation, we studied the effects of ethanol on p42/44 mitogen-activated protein kinase (MAPK), p38 mitogen-activated protein kinase (p38 MAPK) and c-Jun N-terminal kinase (JNK) in normal and regenerating rat liver. Treatment of rat hepatocytes with 100 mM ethanol in vitro for 16 h prolonged the activation of p42/44 MAPK and ...

We recently identified brain-derived neurotrophic factor (BDNF) in the dorsal striatum to be a major component of a homeostatic pathway controlling ethanol consumption. We hypothesized that ethanol-mediated activation of the BDNF signaling cascade is required for the ethanol-related function of the neurotrophic factor. Here, we demonstrate that exposure of striatal neurons to ethanol results in...

Background: The parthenogenetic of oocytes is important in cloning research, as artificial activation of oocytes is an essential component of nucleus transfer protocols. Hydrostatic pressure can act as a mechanical stimulator that rearranges egg contents. Ethanol promotes a single intracellular Ca2+ increase of greater and longer amplitude than the initial increase observed at fertilization. In...

Although ethanol abuse is the most common cause of pancreatitis, the mechanism of alcohol's effect on the pancreas is not well understood. Previously, we demonstrated that in vitro ethanol treatment of pancreatic acinar cells augmented the CCK-8-induced activation of NF-kappaB, a key signaling system involved in the inflammatory response of pancreatitis. In the present study, we determine the r...