PREMISE OF THE STUDY We have developed and optimized microsatellite loci from a genomic library of Erysimum mediohispanicum. Microsatellites were also tested for cross-amplification in 31 other Erysimum species. METHODS AND RESULTS A total of 10 microsatellite loci were successfully amplified. They were polymorphic for 81 E. mediohispanicum individuals from two locations in Sierra Nevada (sou...

Flowers of animal-pollinated plants are integrated structures shaped by the action of pollinator-mediated selection. It is widely assumed that pollination specialization increases the magnitude of floral integration. However, empirical evidence is still inconclusive. In this study, we explored the role of pollinator diversity in shaping the evolution of corolla-shape integration in Erysimum, a ...

Pollinator-mediated evolutionary divergence has seldom been explored in generalist clades because it is assumed that pollinators in those clades exert weak and conflicting selection. We investigate whether pollinators shape floral diversification in a pollination generalist plant genus, Erysimum. Species from this genus have flowers that appeal to broad assemblages of pollinators. Nevertheless,...

Glucosinolates from seed meals of Erysimum hieracifoliwn and l\1alcolmia mantlma were treated with thioglucosidase (EC 3.2.3.1). and the resultant aglucon products investigated. A major product from E. hieracifoliwll was 3-hydroxypropyl isothiocyanate from the novel precursor 3-hydroxypropylglucosinolate. Other aglucon products were 3-methylsulfonylpropyL 3-methylsulfinylpropyl. 3-methylthiopro...

Two new cardiac glycosides were isolated from the seeds of Erysimum cheiranthoides. Their structures were characterized as strophanthidin glycosides of 3-O-alpha-L-rhamnopyranosyl-(1-->4)-3-O-acetyl-beta-D-digitoxopyranosyl and 3-O-beta-D-glucpyranosyl-(1-->4)-alpha-L-rhamnopyranosyl-(1-->4)-3-O-acetyl-beta-D-digitoxopyranosyl.

Paleontological and phylogenetic studies have shown that floral zygomorphy (bilateral symmetry) has evolved independently in several plant groups from actinomorphic (radially symmetric) ancestors as a consequence of strong selection exerted by specialized pollinators. Most studies focused on unraveling the developmental genetics of flower symmetry, but little is known about the adaptive signifi...