Using a sequenced pectate lyase-encoding gene (pel gene), we developed a PCR test for Erwinia carotovora. A set of primers allowed the amplification of a 434-bp fragment in E. carotovora strains. Among the 89 E. carotovora strains tested, only the Erwinia carotovora subsp. betavasculorum strains were not detected. A restriction fragment length polymorphism (RFLP) study was undertaken on the amp...

gene that responds to DNA-damaging agents. J. Bacteriol. 172:3284-3289. McEvoy, J. L., K.K. Thum, and A.K. Chatterjee. 1987. Expression of the E. coli lexA+ gene in Erwinia carotovora subsp, carotovora and its effect on production of pectin lyase and carotovoricin. Fed. European Microbiol. Soc. Microbiol. Lett. 42:205–208. Mount, M.S., P.M. Berman, R.P. Mortlock, and J.P. Hubbard. 1979. Regulat...

Current identification methods for the soft rot erwinias are both imprecise and time-consuming. We have used the 16S-23S rRNA intergenic transcribed spacer (ITS) to aid in their identification. Analysis by ITS-PCR and ITS-restriction fragment length polymorphism was found to be a simple, precise, and rapid method compared to current molecular and phenotypic techniques. The ITS was amplified fro...

Erwinia carotovora subsp. atroseptica (Van Hall) (Eca), Erwinia carotovora subsp. carotovora (Dye) (Ecc), Erwinia chrysanthemi (Burkholder) (Ech) are pathogenic bacterias of potato crop, causing blackleg of stems in the field and soft rot of tubers in storage. During these last years, an outbreak of soft rot potatoes caused by the 3 Erwinia species has been observed in Belgium. Several methods ...

......................................................................................... 9 INTRODUCTION .................................................................................... 10 Perception of the pathogen ................................................................. 11 Gene-for-gene interactions ........................................................ 11 General elicitors ......

The production of extracellular enzymes such as pectate lyase (Pel), polygalacturonase (Peh), cellulase (Cel), and protease (Prt) is activated by the cell density (quorum)-sensing signal, N-(3-oxohexanoyl)-L-homoserine lactone (HSL); plant signals; and aep genes during postexponential growth of Erwinia carotovora subsp. carotovora 71. Studies with mutants of E. carotovora subsp. carotovora 71 d...

The present paper investigates to examine the in vitro antibacterial activities of the chromium nanopartical which preparied by -green synthesis techniquesreduction of potassium dichromate solution with Arachis hypogaea leaf extract containing reducing sugars which act as reducing agent. Chromium nanoparticles were shown to be an effective control of two phytopathogenic bacteria, namely Erwinia...

Erwinia carotovora subsp. betavasculorum strains produced a bactericidal antibiotic in vitro that inhibited a wide spectrum of gram-negative and gram-positive bacteria. The optimum temperature for production was 24 degrees C, and the addition of glycerol to culture media enhanced antibiotic production. Antibiotic production by these strains in the infection court of potato was the principal det...

background & aim:  the aim of this study was to assess the bactericidal effect of three medicinal plant species, including thymus vulgaris, trachyspermum ammi, and mentha aquatica on erwinia carotovora growth. experimental: this research was done in a factorial to completely randomized design with three concentrations, and three replicates on nutrient agar culture medium. results &discu...

Background & Aim:  The aim of this study was to assess the bactericidal effect of three medicinal plant species, including Thymus vulgaris, Trachyspermum ammi, and Mentha aquatica on Erwinia carotovora growth. Experimental: This research was done in a factorial to completely randomized design with three concentrations, and three replicates on nutrient agar c...