Soluble epoxide hydrolase enzyme is a promising therapeutic target for hypertension, vascular inflammation, pain and some other risk factors of cardiovascular diseases. The most potent sEH inhibitors reported in the literature are urea-based ones which often have poor bioavailability. In this study, in a quest for finding potent inhibitors of soluble epoxide hydrolase, some 4,6-disubstituted py...

Soluble epoxide hydrolase enzyme is a promising therapeutic target for hypertension, vascular inflammation, pain and some other risk factors of cardiovascular diseases. The most potent sEH inhibitors reported in the literature are urea-based ones which often have poor bioavailability. In this study, in a quest for finding potent inhibitors of soluble epoxide hydrolase, some 4,6-disubstituted py...

We have analyzed amino acid sequence relationships among soluble and microsomal epoxide hydrolases, haloacid dehalogenases, and a haloalkane dehalogenase. The amino-terminal residues (1-229) of mammalian soluble epoxide hydrolase are homologous to a haloacid dehalogenase. The carboxy-terminal residues (230-554) of mammalian soluble epoxide hydrolase are homologous to haloalkane dehalogenase, to...

inhibitors of soluble epoxide hydrolase (seh) represent one of the novel pharmaceutical approaches for treating hypertension, vascular inflammation, pain and other cardiovascular related diseases. most of the potent seh inhibitors reported in literature often suffer from poor solubility and bioavailability. toward improving pharmacokinetic profile beside favorable potency, two series of 4-benza...

Microsomal epoxide hydrolase, a major antigenic marker of putative preneoplastic hepatocytes was studied in hyperplastic liver nodules and in normal liver tissue. Hyperplastic nodules induced by two different protocols contained 2to 3-fold higher specific activities of microsomal epoxide hydrolase than did normal surrounding tissue. However, no increase in cytosolic epoxide hydrolase determined...

Endogenous, constitutive soluble epoxide hydrolase in mice 3T3 cells was localized via immunofluorescence microscopy exclusively in peroxisomes, whereas transiently expressed mouse soluble epoxide hydrolase (from clofibrate-treated liver) accumulated only in the cytosol of 3T3 and HeLa cells. When the C-terminal lie of mouse soluble epoxide hydrolase was mutated to generate a prototypic putativ...

Microsomal epoxide hydrolase, a major antigenic marker of putative preneoplastic hepatocytes was studied in hyperplastic liver nodules and in normal liver tissue. Hyperplastic nodules induced by two different protocols contained 2to 3-fold higher specific activities of microsomal epoxide hydrolase than did normal surrounding tissue. However, no increase in cytosolic epoxide hydrolase determined...

A rapid and sensitive indirect enzyme-linked immunosorbent assay (ELISA) was developed for microsomal epoxide hydrolase of rat liver. The assay, which is easily and readily performed, is significantly more sensitive than most enzymatic epoxide hydrolase assays routinely used and electroimmunoassays previously developed. The limit of sensitivity of the ELISA is between 2-5 ng of microsomal epoxi...

We have utilized a DNA clone complementary to epoxide hydrolase niRNA as a probe to examine the level of the inRNA in persistent hepatocyte nodules and hepatomas induced by the Solt-Farber chemical carcinogenesis procedure. Epoxide hydrolase inRNA is increased 14-fold in nodules as compared to the level in normal liver. When rats with liver nodules were administered phénobarbital, an inducer o...

The epoxide hydrolase gene from Agrobacterium radiobacter AD1, a bacterium that is able to grow on epichlorohydrin as the sole carbon source, was cloned by means of the polymerase chain reaction with two degenerate primers based on the N-terminal and C-terminal sequences of the enzyme. The epoxide hydrolase gene coded for a protein of 294 amino acids with a molecular mass of 34 kDa. An identica...