background herpes simplex virus type 1 and type 2 (hsv-1 and hsv-2) are common infectious agents worldwide. data on prevalence of hsv-1 and hsv-2 infections are limited in asia, especially in iran. our study aimed on determination of seroepidemiology of hsv-1 and hsv-2 infections in asymptomatic healthy students of islamic azad university of kazeroun, fars province, southwest of iran. patients ...

A B S T R C TELISA (Enzyme-linked immunosorbent assay) is a technique used to assessthe quantification of peptide, protein, antibody and hormone levels, basedon the principle antigen-antibody binding. In ELISA technique, antigenimmobilization will be carried out on solid surface, then bound withantibodies form an bond complex, where complex enzyme. The detection signal in formof color change fo...

total igm, iga, and igm-rheumatoid factor (rf) and iga-rf were quantitated in the serum and synovial fluid of 35 seropositive and 8 seronegative rheumatoid arthritis (ra) patients, using a sensitive enzyme-linked immunosorbent assay (elisa). mononuclear cells isolated from peripheral blood and synovial fluid of three seropositive patients were also stimulated in vitro with phorbol myristate ace...

materials and methods in this descriptive study, 2988 stool samples of patients with acute gastroenteritis were collected from children’s hospitals of 5 main cities of iran. the samples were sent in frozen condition to pediatric infection research center in tehran and stored at -70°c. elisa test was performed for detection of rotavirus antigens. the mean age of study population was 1 to 5 years...

conclusions: it was concluded that the elisa is a suitable method for large scale screening of antibodies against aspergilloma. the easy techniques have shortened the required time of tests (approximately 1.30 hours for our elisa, as opposed to 2.30 hours with commercial elisa kit). this may be a substantial benefit for large numbers of samples that should be tested. materials and methods: an i...

background: nuts are one of the main consumed snacks worldwide and also have an important role among iranian's food habits. natural contamination of nuts with aflatoxin is unavoidable and causes a special challenge for nuts safety and quality. objectives: the purpose of this research was to study the aflatoxin contamination in commercially-available nuts (pistachio, walnut and peanut) in the ma...

b ackground : tuberculosis is a crucial health problem. establishing a rapid, reliable and still inexpensive diagnostic method for tuberculosis seems to be substantial in developing countries where tb has very high incidence rate . methods:   an indirect  enzyme-linked  immunosorbent  assay (elisa )   was established to detect serum antibodies against mycobacterium  tuberculosis. three kinds of...