background herpes simplex virus type 1 and type 2 (hsv-1 and hsv-2) are common infectious agents worldwide. data on prevalence of hsv-1 and hsv-2 infections are limited in asia, especially in iran. our study aimed on determination of seroepidemiology of hsv-1 and hsv-2 infections in asymptomatic healthy students of islamic azad university of kazeroun, fars province, southwest of iran. patients ...

A B S T R C TELISA (Enzyme-linked immunosorbent assay) is a technique used to assessthe quantification of peptide, protein, antibody and hormone levels, basedon the principle antigen-antibody binding. In ELISA technique, antigenimmobilization will be carried out on solid surface, then bound withantibodies form an bond complex, where complex enzyme. The detection signal in formof color change fo...

total igm, iga, and igm-rheumatoid factor (rf) and iga-rf were quantitated in the serum and synovial fluid of 35 seropositive and 8 seronegative rheumatoid arthritis (ra) patients, using a sensitive enzyme-linked immunosorbent assay (elisa). mononuclear cells isolated from peripheral blood and synovial fluid of three seropositive patients were also stimulated in vitro with phorbol myristate ace...

Enzyme-linked immunosorbent assay (ELISA) has been served as both the workhorse and gold standard in immunoassay for its high specificity, sensitivity, accuracy, despite known shortcomings limitations....

Featured Article: Engvall E, Perlmann P. Enzymelinked immunosorbent assay (ELISA). Quantitative assay of immunoglobulin G. Immunochemistry 1971;8:871– 4. This paper was my first as a graduate student of Professor Peter Perlmann at Stockholm University. At the time, RIAs were in full bloom, but they were too sophisticated for many areas of research and diagnosis because they required expensive e...