In line with the recommendations of an ECVAM workshop on reproductive toxicity, held in 1994 (1), ECVAM initiated and funded a validation study of three in vitro embryotoxicity tests. In two of these assays, pregnant laboratory animals are used to obtain embryonic tissue — either primary embryonic cells in the mouse micromass (MM) test or embryos in the rat whole embryo culture (WEC) test. In c...

Rhizophora mangle is an abundant plant in mangroves and tannic acid is a polyphenol produced by the secondary metabolism of plants. The aim of the study was to evaluate the embryotoxic and embriostatic effects of the aqueous extract of R. mangle and synthetic tannic acid on eggs and larvae of Aedes aegypti. A. aegypti eggs were exposed in duplicate at concentrations of 250, 500, 750 and 1000 µg...

Purpose : To 1) evaluate the embryotoxic effects of hydrogen peroxide on mouse embryo development and 2) examine if pentoxifylline can reverse hydrogen peroxide induced embryotoxicity. Methods : Prospective in vitro study examining the effects of varying concentrations of hydrogen peroxide and pentoxifylline on the blastocyst development rate alone as well as in combination. Results : A dose-de...

Peterka M., M. Tu~ek, D. Vesely: EmbryotoxicityoJNineSeedMordants in the Chick Embryo the CHEST Method. Acta vet. Bmo 1996, 65:213-217. The seed mordants Agronal Super, which contains the organic mercury (5.4% phenylmercurychloride), has appeared to induce poisoning and even death of many people and animals. Minimal single embryotoxic doses of Agronal Super were estimated in 2-4-day old chick e...

Backgrounds: A straight-forward way to identify whether a drug or environmental chemical can be harmful to unborn baby is to evaluate its effect on laboratory animals. All invivo methods need large number of animal and are therefore time consuming and expensive. However, the thousands of chemicals in need of testing, to reduce the spending of live animals, an assortment of in vitro assays has ...

backgrounds: a straight-forward way to identify whether a drug or environmental chemical can be harmful to unborn baby is to evaluate its effect on laboratory animals. all invivo methods need large number of animal and are therefore time consuming and expensive. however, the thousands of chemicals in need of testing, to reduce the spending of live animals, an assortment of in vitro assays has b...

The embryonic stem cell test (EST), an alternative model to animal studies, is a reliable and scientifically validated in vitro system for testing embryotoxicity. In contrast to most in vivo animal tests, two permanent cell lines, murine fibroblasts (BALB/c‑3T3 cells) and murine embryonic stem cells (mES‑D3 cells), are used in EST instead of animals in standard tests of toxicity. The embryotoxi...

A survey of the literature indicates that several chemicals whose reduced metabolites are capable of undergoing redox cycling in biological systems also possess significant teratogenic properties when tested in vivo. We have initiated investigations to determine whether the embryotoxic effects of such chemicals could result from their redox cycling properties and whether redox cycling could be ...

The article examines the harm caused by benzapyrene to environment and human health. Being a carcinogen, accumulating in body, it has mutagenic, embryotoxic, hematotoxin effect. Regulations require removal of contaminated soil from construction site with subsequent disposal. disinfection methods are analyzed.

OBJECTIVES Resin (co)monomers may be released from restorative dental materials and can diffuse into the tooth pulp or the gingiva, and can reach the saliva and the circulating blood. Genotoxic potential of some dental composite components has been clearly documented. The genotoxic effects of xenobiotics can represent a possible step in tumor initiation and/or embryotoxicity/teratogenesis. A mo...