Background: Development of cloned bovine embryos. Materials and Methods: Oocytes collection,oocyte denudation, oocyte enucleation, nuclear transfer, electrofusion, reconstructed embryo activation, culture of reconstructed and IVF embryo,and treatment with SAH post fusion interval Treatment of reconstructed embryos with TSA for 12 hours after activation, preparation of somatic donor cells, donor...

background: the selection of pre-embryos for transferred is based on morphological appearance. but some poor quality cleaved embryos also can be cultured to the blastocyst stage and implanted. objective: to assess the clinical pregnancy outcomes of blastocyst transfer which developed from poor quality embryos. materials and methods: a total of 109 cleaved embryos with poor quality were cultured...

during spring, summer and winter seasons, sistani donor cows, with normal reproductive status, were superovulated and embryos were recovered non-surgically on day 7. grade a blastocyst embryos were either transferred fresh (spring) or frozen (summer and winter). recovered embryos during summer and winter were exposed to glycerol and frozen using conventional method. during spring season, recipi...

introduction: mouse embryos can successfully be frozen with sucrose and propandiol. however, different developmental stages of mammalian embryos have shown to have various capacities to undergo freeze-thawing procedure. in the present study, the ability of 1,2,4 and 8-ell mouse embryo to tolerate the freeze-thawing procedure was investigated and the effect of this procedure on the further in vi...

haploid microspore-derived embryos (mdes) of wheat were obtained by in vitro androgenesis. these  embryos were employed to evaluate the transient expression of gus gene (uida) following particle bombardment. using the bio-rad pds-1000/he system, the physical parameters including rupture disk pressure (900, 1100 and 1350 psi); microprojectile travel distance (6 and 9 cm); gold particles size (0....

introduction: the aim of this investigation was to study the developmental potential of human fragmented embryos to blastocyst stage and to evaluate thedeveloped blastocysts in the term of size, cellularity, the type of inner cell mass(icm), trophoetoderm (te) and the number of dead cells. material and methods: four to eight cell fragmented human embryos wereobtained from the embryology lab of ...

background: timing of the first zygotic cleavage is an accurate predictor of embryo quality. embryos that cleaved early (ec) have been shown to exhibit higher developmental viability compared to those that cleaved at a later period (lc). however, the viability of ec embryos in comparison to lc embryos after vitrification is unknown. the present study aims to investigate the post-vitrification d...

Background Timing of the first zygotic cleavage is an accurate predictor of embryo quality. Embryos that cleaved early (EC) have been shown to exhibit higher developmental viability compared to those that cleaved at a later period (LC). However, the viability of EC embryos in comparison to LC embryos after vitrification is unknown. The present study aims to investigate the post-vitrification de...