embryo culture were used to produce inter specific walnut hybrid plants between juglans nigra l. and j. regia l. developed ovaries were collected from j. rejia female flowers which were pollinated with j. nigra pollen grain by high dusting on a mature tree. in order to isolate embryos, exocarps were aseptically removed. isolated embryos from ovules at different developmental stages were transfe...

oocyte maturation and embryo development are controlled by intra-ovarian factors such as steroid hormones. progesterone (p4) exists in the follicular fluid that contributes to normal mammalian ovarian function and has several critical functions during embryo development and implantation, including endometrial receptivity, embryonic survival during gestation and transformation of the endometrial...

introduction: the purpose of this study was to evaluate the effect of different doses of retinoic acid on resumption of meiosis, in vitro maturation of oocytes and resulting embryo development in mice. materials and methods: germinal vesicle of nmri female mouse (6-8 weeks old) oocytes were collected from ovaries and cultured in maturation medium mem-α contained: 100 mliu/ml rfsh + 7.5 iu/ml hc...

Oocyte maturation and embryo development are controlled by intra-ovarian factors such as steroid hormones. Progesterone (P4) exists in the follicular fluid that contributes to normal mammalian ovarian function and has several critical functions during embryo development and implantation, including endometrial receptivity, embryonic survival during gestation and transformation of the endometrial...

Background: In vitro culture of ovarian follicles may preserve fertility in women with premature ovarian failure due to cancer .It seems that creation a condition that could maintain cellular communications and supports growth of follicles to produce mature oocytes appear to be essential. Nitric oxide (NO) has been recently shown to act with a dual action in mouse oocyte meiotic maturation depe...

introduction: the purpose of this study was to evaluated the effect of β-mercaptoethanol on resumption of meiosis, in vitro maturation of immature mouse oocytes and resulting embryo development with and without bso (dl-buthionine sulfoximine). material and methods: germinal vasicle (gv) were recovered from 6-8 weeks old nmri ovaries and cultured in maturation medium in memα supplemented with 7....

introduction: in this study in vitro maturation, resumption of meiosis and development of immature mouse oocytes in the presence of cysteamine was evaluated. meterial and methods: immature oocytes recovered from nmri mouse strain (4-6 weeks) were categorized in three experimental and one control group. the control included 345 immature oocytes which were matured in mem medium contain 5% fcs. in...