nucleocapsid (n) protein of infectious bronchitis virus (ibv), one of the viral structural proteins, inducesstrong antibody response in natural infection. in this study, a simple, recombinant n protein-based dot-blottest was developed to serologically examine chicken serum samples for the presence of ibv antibody.initially, 72 serum samples were tested for the presence of ibv antibody using a c...

Background and Aims: Viroids are smallest known plant pathogens and cause several economically significant diseases. Until recently, viroid detection relied mainly on biological tests and indexing. Today various diagnostic techniques such as nucleic acid hybridization, southern blot and reverse transcription coupled with polymerase chain reaction (RT-PCR) are being used for detection and diag...

background and aims: viroids are smallest known plant pathogens and cause several economically significant diseases. until recently, viroid detection relied mainly on biological tests and indexing. today various diagnostic techniques such as nucleic acid hybridization, southern blot and reverse transcription coupled with polymerase chain reaction (rt-pcr) are being used for detection and diagno...

background and objective: high-level gentamicin resistance (hlgr: mic ≥ 500 µg/ml) in enterococci is mediated by aminoglycoside modifying enzymes which is mainly encoded by aac(6′)-ie-aph(2′′)-ia gene. the aim of this study was to evaluate the frequency of   aac(6′)-ie-aph(2′′)-ia gene in clinical isolates of enterococcus facium and enterococcus faecalis collected from hospitals in northwest of...

In this study, a modified method of the conventional RNA dot-blot hybridization was established, by replacing (32)P labels with CY5 labels and replacing nylon membranes with positive-charged glass slides, for detecting plant RNA viruses and a viroid. The modified RNA dot-blot hybridization method was named glass slide hybridization. The optimum efficiency of RNA binding onto the surfaces of act...