A metal-free synthesis of bifunctionalized indole derivatives was developed through a novel TBHP/TBAI-mediated oxidative coupling of C2,C3-unsubstituted indoles with arylsulfonyl hydrazide. Under the same conditions C3-methyl substituted indoles underwent a diazotization process, affording 2-sulfonyldiazenyl-1H-indoles. The former reaction simultaneously established C-S and C-N bonds through se...

Oxyhemoglobin in erythrocyte hemolysates interferes with the Jendrassik-Grof assay. Destruction of azobilirubin occurs when oxyhemoglobin is oxidized to methemoglobin during diazotization or to alkaline hematin with addition of alkaline tartrate. The most probable mechanism is by oxidation with an agent such as hydrogen peroxide or a related species resulting from hemoglobin oxidation. Methemog...

The main goal of this research was the synthesis of enantiopure R(-)-3-aminoisobutyric acid from dihydrothymine with good yield, high stereospecificity and relative simplicity. Seventy two percent yield of the product was obtained in three steps. Step one consisted of dihydrothymine racemization. Step two was a dihydropyrimidinase reaction involving the Pseudomonas aeruginosa 10145 bacterial st...

The field of DNA microarray technology has necessitated the cooperative efforts of interdisciplinary scientific teams to achieve its primary goal of rapidly measuring global gene expression patterns. A collaborative effort was established to produce a chemically reactive surface on glass slide substrates to which unmodified DNA will covalently bind for improvement of cDNA microarray technology....

A highly sensitive and accurate spectrophotometric method including Technique ( Normal Flow injection analysis have been used for determined Nifedipine (NIF) drug in pure dosage forms. This approach based on the response of with diazotized sulfamethoxazole (SMZ) to form colored product after diazotization reagent coupling reaction The coloured chromogen suggests absorption maximum at 496 nm. li...

Simple and accurate spectrophotometric methods have been developed for the determination of lamotrigine in tablet formulation. The method A was based on the diazotization of lamotrigine followed by coupling with 4-(dimethylamino)benzaldehyde in acid medium to give greenish yellow product with a λmax of 410 nm. The method B also was based on the diazotization of drug and conversion of phenol int...

a convenient, rapid, and one-pot method for the synthesis of azo dyes has been developed. in this protocol, diazotization reagent (arn2+ -cro3–sio2) was prepared via grinding of aromatic amines, nano2, wet sio2 and nano silica chromic acid (nano-sca) without solvent at room temperature. the obtained diazotization reagent was sufficiently stable to be kept at room temperature in the dry state fo...