Norovirus is a major cause of viral gastroenteritis, with shellfish consumption being identified as one potential norovirus entry point into the human population. Minimising shellfish norovirus levels is therefore important for both the consumer's protection and the shellfish industry's reputation. One method used to reduce microbiological risks in shellfish is depuration; however, this process...

Low levels of feces-associated natural virus, simulating virus numbers estimated to exist in moderately polluted shellfish-growing waters, were used to evaluate the effectiveness of depuration as a virus depletion procedure in soft-shell clams. Depuration effectiveness depended upon the numbers of virus bioaccumulated and whether virus was solids associated. Virus uptake was greatest when virus...

This study presents a series of short-term studies (total duration 48 h) of uptake and depuration of engineered nanoparticles (ENP) in neonate Daphnia magna. Gold nanoparticles (Au NP) were used to study the influence of size, stabilizing agent and feeding on uptake and depuration kinetics and animal body burdens. 10 and 30 nm Au NP with different stabilizing agents [citrate (CIT) and mercaptou...

The present study was performed to determine the depuration time and ability of Donax trunculus (Wedge Clam) and Tapes decussatus (Carpet Shell) contaminated with Escherichia coli, Salmonella enterica subsp. enterica serovar Typhimurium and Vibrio parahaemolyticus. Clams were contaminated with each bacterium at the level of 7.0 - 8.0 Log10 cfu/g. After contamination, clams were analyzed every 3...

Since kNN classifiers are sensitive to outliers and noise contained in the training data set, many approaches have been proposed to edit the training data so that the performance of the classifiers can be improved. In this paper, through detaching the two schemes adopted by the Depuration algorithm, two new editing approaches are derived. Moreover, this paper proposes to use neural network ense...

Rainbow trout were exposed for 72 h to [ 14C]anthracene alone and [ I4C]anthracene in an oil shale retort water. Tissue levels of [I4C]anthracene were analyzed at 24, 48 and 72 h to determine non-steady-state bicconcentration factors (BCFs), and uptake and depuration rates were calculated from anthracene disappearance in exposure waters and metabolite appearance in depuration waters. Uptake rat...

When filter-feeding shellfish are consumed raw, because of their ability to concentrate and store waterborne pathogens, they are being increasingly associated with human gastroenteritis and have become recognized as important pathogen vectors. In the shellfish industry, UV depuration procedures are mandatory to reduce pathogen levels prior to human consumption. However, these guidelines are bas...

Within the country of Brazil, Santa Catarina is a major shellfish producer. Detection of viral contamination is an important step to ensure production quality and consumer safety during this process. In this study, we used a depuration system and ultraviolet (UV) disinfection to eliminate viral pathogens from artificially infected oysters and analysed the results. Specifically, the oysters were...

The depuration effectiveness of Poliovirus 1 and HAV contaminated mussels, when subjected to flowing ozonized water, was evaluated. Mussels were experimentally contaminated and then depurated up to 72 hours using a pilot depuration system with ozone treated and untreated water. The results showed that viral infectivity titter decreased more rapidly in mussels subjected to ozonized water (from 1...

We carried out uptake and depuration experiments in the laboratory to investigate the effects of temperature (15 degrees C and 25 degrees C) on the depuration kinetics and persistence of a cyanobacterial toxin, microcystin-LR (MCYST-LR), in a freshwater bivalve, Unio douglasiae. Bivalves were fed toxic Microcystis cells in the 15-day uptake experiment and nontoxic diatoms in the following 15-da...