AIMS To develop an in vitro model (Colony/drip-flow reactor - C/DFR) for the growth and analysis of methicillin-resistant Staphylococcus aureus (MRSA) biofilms. METHODS AND RESULTS Using the C/DFR model, biofilms were grown on the top of polycarbonate filter membranes inoculated with a clinical isolate of MRSA, placed on absorbent pads in the DFR and harvested after 72 h. The biofilms varied ...

Cultured skin grafts administered clinically for closure of burn wounds may be contacted by topically applied antimicrobial agents. A study was performed to assess whether commonly used topical antimicrobial agents are toxic to cultured human keratinocytes (HK) in vitro. Serum-free MCDB 153 culture medium containing Neosporin G.U. irrigant (Neomycin, 40 micrograms/ml-polymyxin B sulfate, 200 un...

INTRODUCTION Although the use of negative pressure wound therapy (NPWT) is broadly efficacious, it may foster some potentially adverse complications. This is particularly true in patients with diabetes who have a wound colonized with aerobic organisms. Traditional antiseptics have been proven useful to combat such bacteria but require removal of some NPWT devices to be effective. METHODS In t...

in this paper, we investigate the existence of positive solutions for the ellipticequation $delta^{2},u+c(x)u = lambda f(u)$ on a bounded smooth domain $omega$ of $r^{n}$, $ngeq2$, with navier boundary conditions. we show that there exists an extremal parameter$lambda^{ast}>0$ such that for $lambda< lambda^{ast}$, the above problem has a regular solution butfor $lambda> lambda^{ast}$, the probl...

we consider the semilinear elliptic boundary value problem  = ∈∂ω− δ = ∈ωu x xu x f u x x( ) 0;( ) λ ( ( ));where λ > 0 is a parameter, ω is a bounded region in rn with a smooth boundary, and f is asmooth function. we prove, under some additional conditions, the existence of a positive solution for λlarge. we prove that our solution u for λ large is such that = →∞∈ω|| u ||: sup| u(x) |xas λ ...