Previously, we reported that the 3,4-epoxypiperidine structure, whose design was based on the active site of DNA alkylating antitumor antibiotics, azinomycins A and B, possesses prominent DNA cleavage activity. In this report, novel caged DNA alkylating agents, which were designed to be activated by UV irradiation, were synthesized by the introduction of four photo-labile protecting groups to a...

Posttranslational modifications (PTMs), such as phosphorylation and ubiquitination, play critical regulatory roles in the assembly of DNA damage response proteins on the DNA damage site and their activities in DNA damage repair. Tyrosyl DNA phosphodiesterase 2 (TDP2) repairs Topoisomerase 2 (Top2)-linked DNA damage, thereby protecting cancer cells against Top2 inhibitors-induced growth inhibiti...

Herbal infusions of nine traditionally used medicinal plants were screened for total phenolic content (TPC), radical scavenging activity (RSA) and oxidative DNA damage protecting capacity by chemical assays. The TPC was determined in order to know its correlation with RSA. The extracts of T. cordifolia and C. spectabilis presented highest TPC values (34.21 ± 0.93 and 28.36 ± 1.05 mg GAE/g) and ...

OBJECTIVE To analyze the total phenolic content, DNA protecting and radical scavenging activity of ethanolic leaf extracts of three Lamiaceae plants, i.e. Anisomelos malabarica (A. malabarica), Leucas aspera (L. aspera) and Ocimum basilicum (O. basilicum). METHODS The total polyphenols and flavonoids were analyzed in the ethanolic leaf extracts of the lamiaceae plants. To determine the DNA pr...

In this study, the antioxidant, antiacetylcholinesterase, anti-inflammatory, and DNA protecting activities of the aerial parts of Glaucium grandiflorum var. grandiflorum were evaluated. It was found that the lyophilized methanolic extract exhibited an inhibitory capacity against FeCl3/ascorbic acid induced posphatidylcholine liposome oxidation (EC50 = 2.62  0.08 mg/mL), quenched HOCl (5.87  0...

Faithful preservation of genome integrity is the critical mission of stem cells as well as of germ cells. Reviewed are the following mechanisms involved in protecting DNA in these cells: (a) The efflux machinery that can pump out variety of genotoxins in ATP-dependent manner; (b) the mechanisms maintaining minimal metabolic activity which reduces generation of reactive oxidants, by-products of ...