background and aims: glycoprotein b (gb) is among the most important immunogenic proteins of hsv-1 that is now being used as recombinant vaccine to induce protectively against the virus. it is believed that gb is responsible for induction of neutralizing antibody and up to 90% of ctl (cytotoxic t cell) activity against hsv-1 in some haplotypes. methods: in the present study the antibody titer i...

conclusions the pc-ns3 possesses the capacity to express ns3 in the mammalian cell line and demonstrated strong immunogenicity in a murine model. our primary results demonstrated that the immunogenic truncated region of ns3 could be used as a potential vaccine candidate against hepatitis c. results the pcdna3.1 plasmid harboring the coding sequence of ns3 (pc-ns3) was constructed and confirmed ...

Immunization with purified DNA is a powerful technique for inducing immune responses. The concept of DNA immunization involves insertion of the gene encoding the antigen of choice into a bacterial plasmid and injection of the plasmid into the host where the antigen is expressed and where it induces humoral and cellular immunity. The most effective routes and methods for DNA immunization are bom...

Background: DNA immunization is an appropriate method to produce an immunological response. Pseudomonas aeruginosa produces exotoxin A which is highly cytotoxic for eukaryotic cells. Since domains II (translocation domain) and 1b of the toxin have antigenic qualities, so they could be  useful candidates to protect against pseudomonas infections. Objectives: To evaluate if recombinant plasmid co...

Immunization of experimental autoimmune encephalomyelitis (EAE)-prone C57BL/6 mice with MOG35-55 (a model used to study aspects of human multiple sclerosis) is known to lead to the production of various abzymes. The production of catalytic IgGs that can efficiently hydrolyse myelin basic protein (MBP), MOG and DNA is associated with changes in the profile of differentiation and level of prolife...

The immune response induced by immunization with HIV Env DNA and virus-like particle (VLP) vaccines was investigated. Immunization with the HIV Env DNA vaccine induced a strong CD8 T cell response but relatively weak antibody response against the HIV Env whereas immunization with VLPs induced higher levels of antibody responses but little CD8 T cell response. Interestingly, immunization with a ...

Immunizations of mice with plasmid DNAs encoding ovalbumin (OVA), human Ig, and hen egg lysozyme were compared with doses of soluble protein (without adjuvant) that induced similar IgG responses. The route of immunization influenced the magnitude of the antibody (Ab) response in that intradermal (i.d.) injection elicited higher IgG Ab levels than i.m. injection in both DNA- and protein-immunize...