in this study, we explained a nanobiosensor for dna sequence detection, featuring sequence specificity, cost efficiency, speed, and ease of use. without the need for labels or indicators, it may be ideal for the detection of biological agents. this review describes recent advances in electrochemical impedance spectroscopy (eis) with an emphasis on using nanoparticles and nanotechnology. a power...

the transformation of biochemical information into a physical or chemical signal is the basic idea behind a biosensor. the efficient detection of charged biomolecules by biosensor with appropriate device has caught tremendous research interest in the present decade. the present work is related to the simulation study of the performance of a functionalized surface of a biologically sensitive fie...

The glass surface modification with 3-(mercaptopropyl) trimethoxysilane (MPTS), gold nano-particles (GN) and guanine rich single strand DNA (ss-DNA) was utilized as a novel and efficient platform for sensing trace concentration of methylene blue (MB) by an inexpensive spectrophotometric method. Methylene Blue (MB) can interact with the guanine base of single strand DNA and absorbed onto glass s...

The glass surface modification with 3-(mercaptopropyl) trimethoxysilane (MPTS), gold nano-particles (GN) and guanine rich single strand DNA (ss-DNA) was utilized as a novel and efficient platform for sensing trace concentration of methylene blue (MB) by an inexpensive spectrophotometric method. Methylene Blue (MB) can interact with the guanine base of single strand DNA and absorbed onto glass s...

Ultrasensitive molecular beacon (MB) DNA biosensors, with micrometer to submicrometer sizes, have been developed for DNA/RNA analysis. The fluorescence-based biosensors have been applied in DNA/ RNA detection without the need for a dye-labeled target molecule or an intercalation reagent in the testing solution. Molecular beacons are hairpin-shaped oligonucleotides that report the presence of sp...

Objective: With the increasing incidence of cancer and the dramatic effect of early detection on treatment and increase patient's life, many efforts have been devoted to making sensitive diagnosis systems. DNA as a biomarker for diagnosis of different types of cancers at the early stages of illness has attracted much attention.Methods: In this research novel electrochemical biosensor was ...

An ultrasensitive electrochemical biosensor for the determination of pathogenic Vibrio cholerae (V. cholerae) DNA was developed based on polystyrene-co-acrylic acid (PSA) latex nanospheres-gold nanoparticles composite (PSA-AuNPs) DNA carrier matrix. Differential pulse voltammetry (DPV) using an electroactive anthraquninone oligonucleotide label was used for measuring the biosensor response. Loa...

We have developed a colorimetric biosensor using a dual platform of gold nanoparticles and graphene oxide sheets for the detection of Salmonella enterica. The presence of the invA gene in S. enterica causes a change in color of the biosensor from its original pinkish-red to a light purplish solution. This occurs through the aggregation of the primary gold nanoparticles-conjugated DNA probe onto...

Here we report the development of a whole-cell biosensor to detect and quantify the induction of the SOS response activated by DNA-degrading colicins. This biosensor utilizes the SOS-responsive cda promoter to regulate the expression of green fluorescent protein. The biosensor assay revealed induction of stress for all DNA-degrading reference colicins (E2, E7, and E8).