medicinal plants have high importance in medicine and pharmacogonosy because of their secondary metabolites. essential oils, antioxidants and flavonoids are the major compounds of many medicinal plants. these compounds especially antioxidants can increase oxidation of dna in response to wounds. nowadays, progresses in biotechnology such as molecular markers were used for phylogenetic relationsh...

background: isolation of high quality genomic dna is one of the most important steps in molecular biology studies related to food borne parasites. usually, various protocols are used for different tissues but so far, there is no common, simple and cost benefit procedure for genomic dna extraction of linguatula serrata ( l. serrata .) larvae as a pentastomida endangering food safety. one of the ...

background: infestation of the skin by the “itch mite” sarcoptes scabiei var. hominis results in a contagious skin infection in humans called “sca-bies”. by resolving morphology issues, the present study was designed to be acquainted with itch mite by molecular markers. methods: the mite samples were collected from scabies patients by visiting government hospitals of twin city, pakistan. for su...

background: extraction of blood genomicdnais one of the main approaches for clinical and molecular biology studies. although several methods have been developed for extraction of blood genomic dna, most of these methods consume long time and use expensive chemicals such as proteinase k and toxic organic solvent such as phenol and chloroform. the objective of this study was to developed easy and...

colorectal cancer (crc) is one of the most common forms of cancers in the world and is curable if diagnosed at the early stage. analysis of dna extracted from stool specimens is a recent advantage to cancer diagnostics. many protocols have been recommended for dna extraction from stool, and almost allof them are difficult and time consuming, dealing with high amount of toxic materials like phen...

objective: in this study, the possibility of prenatal diagnosis of down syndrome with real-time pcr method was evaluated. in this context, optimization of a suitable method for purification of high quality dna from amniotic fluid samples was also considered. materials and methods: pregnant women who had the high risk of having babies with down syndrome were selected according to the biochemica...

nosocomial infection caused by methicillin-resistant staphylococci poses a serious problem in many countries. the aim of this study was to rapidly and reliably detect methicillin-resistant-staphylococci in order to suggest appropriate therapy. the presence or absence of the methicillin-resistance gene in 115 clinical isolates of staphylococcus aureus and 50 isolates of coagulase negative staphy...

background :  sequence-based identification of various microorganisms including archaea, bacteria, cyanobacteria, diatoms, fungi, and green algae necessitates an efficient and reproducible genome extraction procedure though which a pure template dna is yielded and it can be used in polymerase chain reactions (pcr). considering the fact that dna extraction from these microorganisms is time consu...

a total of ten isolates of fungi with rhizoctonia-like mycelia were obtained from infected roots and stems of pistachio pistacia vera grown in a commercial nursery in rafsanjan, iran, during the autumn of 2011. the infected seedlings showed symptoms of chlorosis that later turned to necrosis. all of the isolates were identified as binucleate rhizoctonia on the basis of hyphal characteristics an...

background: cystic hydatid disease is an important zoonosis, affecting humans and animals and is a significant public health and economic problem throughout the world and iran. since extraction of dna from the parasite is a primary and crucial step which has a principal effect on pcr results, in the current study five simple methods for dna extraction from protoscoleces of echinococcus granulos...