abstract in this experiment the effects of two type of medium cultures, gibberellic acid and dark/cold and light/warm culture conditions on mature embryos germination of a local cultivar of walnut were investigated. the mature fruits were collected at 22 weeks postanthesis and after disinfection the mature embryo along with cotyledon pieces were excised and then were cultured on mt and modified...

A complete micropropagation protocol was developed for Pistacia vera cv. Ghazvini, an important rootstock in Pistachio orchards in Iran. In present study, the efficiency of a new medium called GNH (Garoosi, Nezami and Haddad) was investigated against some standard media. Different vitamins, calcium (Ca) sources and plant growth regulators (PGRs) were tested on in vitro shoot multiplication and ...

SummaryAim. To optimize the processes of microclonal propagation Juglans regia in vitro by selecting composition and consistency nutrient medium. Methods. The methods culture establishment initial explants were used. Parts stem buds young sprouts, germinated mini-greenhouse method from seeds regia, used as explants. Murashige&Skoog (MS) Driver&Kuniyuki (DKW) media to establish vitro. Ag...

Effects of various culture media and growth regulators on micropropagation of cherry rootstock, M × M60 were investigated in the present study. Stem cuttings of the current season growth were taken from mature stockplants and cultured on the establishment medium after disinfection. After four weeks, elongated shoots were cut and subcultured on the proliferation media including MS, DKW, QL and M...

Juniperus drupacea Labill. (Cupressaceae) is a species with ecological and medicinal value. In Europe, it native only in southern Greece, listed as endangered. Due to its uniqueness, this study attempted, for the first time, an vitro propagation effort of Syrian juniper. Explants lateral shoot tips were surface-sterilized cultured on Murashige Skoog (MS) medium. The cultures subcultured MS, woo...

Micropropagation of hemp ( Cannabis sativa ) is constrained by problems with hyperhydricity and culture decline microshoots. These can be reduced increasing agar nutrients in the media during micropropagation stages 1 2, respectfully. Performance microshoots ‘Abacus’ ‘Wife’ cultured Driver Kuniyuki Walnut medium (DKW) for 15 weeks (6 stage + 9 2), subculturing every 3 both or Murashige Skoog vi...

The effect of nephronic mass reduction of kidney transplants has not been analyzed specifically in a large cohort. Transplant injuries in cadaver kidney graft may have led to an underestimation of the magnitude of this factor. The aim of this study was to analyze the consequences of kidney mass reduction on transplantation outcome. The weights of 1142 kidney grafts were collected prospectively ...

this research was conducted to study the factors affecting establishment, growth and shoot proliferation of persian walnut, genotype z60. explant establishment was studied through a factorial experiment based on a completely randomized design with two factors including culture media (dkw and wpm) and ba concentration (0 (control), 0.5 and 1.0 µm). single-node explants were prepared from the you...