A complete micropropagation protocol was developed for Pistacia vera cv. Ghazvini, an important rootstock in Pistachio orchards in Iran. In present study, the efficiency of a new medium called GNH (Garoosi, Nezami and Haddad) was investigated against some standard media. Different vitamins, calcium (Ca) sources and plant growth regulators (PGRs) were tested on in vitro shoot multiplication and ...

A prerequisite for most plant transformation systems is an efficient and reliable method to regenerate phenotypically normal plants. The present studies were undertaken with a view to the effects of Benzyl amino purine (BAP) and Indol butyric acid (IBA) hormones and genotypes on in vitro germination of immature embryos in walnut (Juglans regia L.). The immature embryos of walnut were excised fr...

abstract in this experiment the effects of two type of medium cultures, gibberellic acid and dark/cold and light/warm culture conditions on mature embryos germination of a local cultivar of walnut were investigated. the mature fruits were collected at 22 weeks postanthesis and after disinfection the mature embryo along with cotyledon pieces were excised and then were cultured on mt and modified...

Pruacan is a rare medicinal plant endemic to the Dieng Plateau of Central Java, Indonesia which has properties as an aphrodisiac. Increasing genetic variability ofthis in order support breeding programs and increase productivity active ingredients this important. This study aimed evaluate effect chemical mutagen EMS on growth development vitro culture pruacan lethal dose determination. Embryoge...

SummaryAim. To optimize the processes of microclonal propagation Juglans regia in vitro by selecting composition and consistency nutrient medium. Methods. The methods culture establishment initial explants were used. Parts stem buds young sprouts, germinated mini-greenhouse method from seeds regia, used as explants. Murashige&Skoog (MS) Driver&Kuniyuki (DKW) media to establish vitro. Ag...

Effects of various culture media and growth regulators on micropropagation of cherry rootstock, M × M60 were investigated in the present study. Stem cuttings of the current season growth were taken from mature stockplants and cultured on the establishment medium after disinfection. After four weeks, elongated shoots were cut and subcultured on the proliferation media including MS, DKW, QL and M...

Juniperus drupacea Labill. (Cupressaceae) is a species with ecological and medicinal value. In Europe, it native only in southern Greece, listed as endangered. Due to its uniqueness, this study attempted, for the first time, an vitro propagation effort of Syrian juniper. Explants lateral shoot tips were surface-sterilized cultured on Murashige Skoog (MS) medium. The cultures subcultured MS, woo...

Micropropagation of hemp ( Cannabis sativa ) is constrained by problems with hyperhydricity and culture decline microshoots. These can be reduced increasing agar nutrients in the media during micropropagation stages 1 2, respectfully. Performance microshoots ‘Abacus’ ‘Wife’ cultured Driver Kuniyuki Walnut medium (DKW) for 15 weeks (6 stage + 9 2), subculturing every 3 both or Murashige Skoog vi...