This vignette provides a tutorial for applying the Discriminant Analysis of Principal Components (DAPC [1]) using the adegenet package [2] for the R software [3]. This methods aims to identify and describe genetic clusters, although it can in fact be applied to any quantitative data. We illustrate how to use find.clusters to identify clusters, and dapc to describe the relationships between thes...

This vignette provides a tutorial for applying the Discriminant Analysis of Principal Components (DAPC [1]) using the adegenet package [2] for the R software [3]. This methods aims to identify and describe genetic clusters, although it can in fact be applied to any quantitative data. We illustrate how to use find.clusters to identify clusters, and dapc to describe the relationships between thes...

Genetic defects in the dystrophin-associated protein complex (DAPC) are responsible for a variety of pathological conditions including muscular dystrophy, cardiomyopathy, and vasospasm. Conserved DAPC components from humans to Caenorhabditis elegans suggest a similar molecular function. C. elegans DAPC mutants exhibit a unique locomotory deficit resulting from prolonged muscle excitation and co...

DP71 is the major cerebral dystrophin isoform and exerts its multiple functions via the dystrophin-associated protein complex (DAPC), also comprised of b-dystroglycan (b-DG) and a1-syntrophin (a1-Syn). Since DP71 disruption leads to impairment in the central control of the osmoregulatory axis, we investigated: 1) the DAPC composition in the hypothalamic supraoptic nucleus (SON) and paraventricu...

This vignette provides a tutorial for applying the Discriminant Analysis of Principal Components (DAPC [1]) using the adegenet package [2] for the R software [3]. This methods aims to identify and describe genetic clusters, although it can in fact be applied to any quantitative data. We illustrate how to use find.clusters to identify clusters, and dapc to describe the relationships between thes...

Platelets are dynamic cell fragments that modify their shape during activation. Utrophin and dystrophins are minor actin-binding proteins present in muscle and non-muscle cytoskeleton. In the present study, we characterised the pattern of Dp71 isoforms and utrophin gene products by immunoblot in human platelets. Two new dystrophin isoforms were found, Dp71f and Dp71 d, as well as the Up71 isofo...

The precise functional role of the dystrophin 71 in neurons is still elusive. Previously, we reported that dystrophin 71d and dystrophin 71f are present in nuclei from cultured neurons. In the present work, we performed a detailed analysis of the intranuclear distribution of dystrophin 71 isoforms (Dp71d and Dp71f), during the temporal course of 7-day postnatal rats hippocampal neurons culture ...

DP71 is the major cerebral dystrophin isoform and exerts its multiple functions via the dystrophin-associated protein complex (DAPC), also comprised of β-dystroglycan (β-DG) and α1-syntrophin (α1-Syn). Since DP71 disruption leads to impairment in the central control of the osmoregulatory axis, we investigated: 1) the DAPC composition in the hypothalamic supraoptic nucleus (SON) and paraventricu...

Dystrophin is an essential component in the assembly and maintenance of the dystrophin-associated protein complex (DAPC), which includes members of the dystroglycan, syntrophin, sarcoglycan and dystrobrevin protein families. Distinctive complexes have been described in the cell membrane of different tissues and cultured cells. In this work, we report the identification and characterization of a...

The lethal muscle-wasting disorder, Duchenne muscular dystrophy, is caused by mutations or deletions in the dystrophin gene. In skeletal and cardiac muscle, dystrophin associates with various proteins to form the dystrophinassociated protein complex (DAPC). The DAPC is thought to play a structural role in linking the actin cytoskeleton to the extracellular matrix, stabilizing the sarcolemma dur...