it has been proposed that organic nitrates such as glyceryl trinitrate (gtn), used in the treatment of cardiovascular diseases, act by producing nitric oxide (no). however, the biochemical pathway for no formation from gtn is not well understood. in the present study, we showed that nitrate formation from gtn, by isolated rat hepatocytes, was inhibited about 50% when cellular glutathione was de...

addition of n-butyl nitrite to isolated rat hepatocytes caused an immediate glutathione depletion followed by an inhibition of mitochondrial respiration, inhi- bition of glycolysis and atp depletion. at cytotoxic butyl nitrite concentrations, lipid  peroxidation  occurred  before  the  plasma  membrane  was  disrupted. cytochrome p-450 inhibitors inhibited peroxynitrite formation and prevented ...

      Addition of n-butyl nitrite to isolated rat hepatocytes caused an immediate glutathione depletion followed by an inhibition of mitochondrial respiration, inhi- bition of glycolysis and ATP depletion. At cytotoxic butyl nitrite concentrations, lipid  peroxidation  occurred  before  the  plasma  membrane  was  disrupted. Cytochrome P-450 inhibitors inhibited peroxynitrite formation and prev...

Frisbee, Jefferson C., John R. Falck, and Julian H. Lombard. Contribution of cytochrome P-450 v-hydroxylase to altered arteriolar reactivity with high-salt diet and hypertension. Am J Physiol Heart Circ Physiol 278: H1517–H1526, 2000.—The present study evaluated the contribution of cytochrome P-450 v-hydroxylase in modulating the reactivity of cremaster muscle arterioles in normotensive rats on...

The oxidation of ethanol by a reconstituted system containing NADPH-cytochrome P-450 reductase and cytochrome P-450 from phenobarbital-treated rats was characterized especially with regard to the role of oxygen radicals in the mechanism of ethanol oxidation. Results described in the preceding manuscript demonstrated that a strong oxidizing species with properties similar to that of the hydroxyl...

The aromatization of androstenedione is inhibited in the presence of aminoglutethimide, diethylaminoethyl-2,2-diphenyl valerate HCl (SKF 525-A), and 1W3 M KCN, but not carbon monoxide or metyrapone. The role of cytochrome P-450 in this reaction could not, therefore, be unequivocally established by inhibition studies. However, it was determined that steroid binding to placental microsomal cytoch...

By using 3-amino-1,2,4-triazole, an inhibitor of haem synthesis, and 2-allyl-2-isopropylacetamide, a drug that degrades the haem moiety of cytochrome P-450, the involvement of haem in cytochrome P-450 synthesis and assembly was investigated. Phenobarbital was used to stimulate apo-(cytochrome P-450) synthesis. Degradation of preformed cytochrome P-450 haem does not result in a concomitant relea...

Somatic cell hybrids were made between mouse myeloma cells and spleen cells derived from BALB/c mice immunized with liver microsomal cytochrome P-450 purified from rats treated with 3-methylcholanthrene (MC-P-450). Thirty-seven independent hybrid clones among 66 tested produced mono clonal antibodies to the MC-P-450 as measured by radioimmunoassay. More than 10 of the monoclonal antibodies form...