A homogeneous preparation of cyclic CMP phosphodiesterase (Helfman, D. M., Shoji, M., and Kuo, J. F. (1981) J. Biol. Chem. 256, 6327-6334) was found to catalyze the hydrolysis of both pyrimidine and purine cyclic 2':3'- and 3':5'-nucleotides. Hydrolysis of cyclic 2':3'-nucleotides resulted in the formation of both 2'- and 3'-nucleotides, although relative amounts of the products were variable. ...

Human peripheral blood monocytes have been found to undergo a transitory state of high accessory activity before they fully become macrophages. Time kinetics were done to follow this accessory potential. Studying the regulation of accessory activity, we have found that monocyte derived accessory cells (m-AC) pass through two phases of development, both of which are adversely controlled by ...

human peripheral blood monocytes have been found to undergo a transitory state of high accessory activity before they fully become macrophages. time kinetics were done to follow this accessory potential. studying the regulation of accessory activity, we have found that monocyte derived accessory cells (m-ac) pass through two phases of development, both of which are adversely controlled by cycli...

The enthalpy of hydrolysis of the 3’-bond of several cyclic 3’,5’-nucleotides and of the 2’-bond of several cyclic 2’,3’nucleotides has been measured calorimetrically. In each case, the enthalpy was indicative of a high energy bond, although values for different compounds varied from -7.7 to 14.1 kcal mole-‘. The enthalpy of hydrolysis of the cyclic 3’,5’-nucleotides, but not that of the cyclic...

Sensitive and specific radioimmunoassays have been developed for cyclic adenosine 3’, 5’-monophosphate (CAMP), cyclic guanosine 3’ ,5’-monophosphate (cGMP), cyclic inosine 3’ ,5’-monophosphate (cIMP), and cyclic uridine 3’, 5’monophosphate (cUMP). These assays are based upon competition of the cyclic nucleotide with isotopically labeled cyclic nucleotide derivatives for binding sites on specifi...

The enthalpy of hydrolysis of the 3’-bond of several cyclic 3’,5’-nucleotides and of the 2’-bond of several cyclic 2’,3’nucleotides has been measured calorimetrically. In each case, the enthalpy was indicative of a high energy bond, although values for different compounds varied from -7.7 to 14.1 kcal mole-‘. The enthalpy of hydrolysis of the cyclic 3’,5’-nucleotides, but not that of the cyclic...