Background: Sperm storage via freezing has been useful for men who have difficulty masturbating during assisted reproductive technology (ART) programs and before impotency caused by chemotherapy, vasectomy, and other procedures. Studies were undertaken to evaluate the extent of cryoinjury to both semen and sperm after repeated freezing and thawing in oligozoospermic and normozoospermic samples ...

An improvement in avian semen cryopreservation is essential and has the potential to improve the cryo-gene banking efficiency. This study compared two cryopreservation methods (slow freezing and vitrification) and the effect of different thawing/warming temperatures (5 ̊C, 25 ̊C and 41 ̊C) on Venda cockerel’s spermatozoa. Semen samples from Venda cockerels were diluted with modified Kobidil extend...

There have been several recent incidents involving the exploding of cryopreservation vials in Trust and Medical School laboratories. This occurs when liquid nitrogen (LN ) leaks into the vials and rapidly expands when removed from the tank. In one incident in our Medical School a research worker’s hand was injured. At Edinburgh University the lid of a cryovial was projected into a student’s eye...

BACKGROUND We designed this study to detect the cryoinjury rate on human sperm after serial freezing and thawing, taking into consideration the effects of using cryovials and straws. MATERIALS AND METHODS In this experimental study, semen specimens obtained from 15 subjects were divided into normozoospermic and oligozoospermic groups. Each of the normozoospermic and oligozoo spermic semen spe...

The standardized assessments of HIV-specific immune responses are of main interest in the preclinical and clinical stage of HIV-1 vaccine development. In this regard, HIV-1 Env-pseudotyped viruses play a central role for the evaluation of neutralizing antibody profiles and are produced according to Good Clinical Laboratory Practice- (GCLP-) compliant manual and automated procedures. To further ...

Periodontitis is inflammation of the periodontal tissue that can cause progressive damage to ligament and alveolar bone. Clinical examination determine degree bone using a probe instrument or probing. Cellular activity in gingival crevicular fluid thought be biomarker inflammatory status tissue. immune responses suggest role cytokines pathogenesis gingivitis periodontitis. The observed this stu...

A current focus of biopharmaceutical research and production is seed train process intensification. This allows for intermediate cultivation steps to be avoided or even the direct inoculation a bioreactor with cells from cryovials cryobags. Based on preliminary investigations regarding suitability high cell densities cryopreservation perfusion cultivations as inoculum further cultivations, an u...

The development of efficient and robust methods for the cryopreservation of human embryonic stem cells (hESCs) is important for the production of master and working cell banks for future clinical applications. Such methods must meet requirements of good manufacturing practice (GMP) and maintain genetic stability of the cell line. We investigated the culture of four Shef hESC lines in gas permea...