Crown gall tumors develop after integration of the T-DNA of virulent Agrobacterium tumefaciens strains into the plant genome. Expression of the T-DNA-encoded oncogenes triggers proliferation and differentiation of transformed plant cells. Crown gall development is known to be accompanied by global changes in transcription, metabolite levels, and physiological processes. High levels of abscisic ...

Renaturation kinetics of labeled Agrobacterium tumefaciens DNA are not influenced by addition of 10(4)-fold excess of crown gall tumor DNA. Reconstruction experiments demonstrated that 0.01% added bacterial DNA produces a detectable increase in rate of renaturation of labeled DNA. Crown gall tumor DNA therefore cannot contain as much as 0.01% A. tumefaciens DNA (one entire bacterial genome per ...

Six different strains of crown gall bacteria were tested to determine whether the commonly used biocontrol strain, K-84, would present them from infecting the paradox hybrid. Previous research showed that K-84 was highly effective in preventing crown gall infections of English root stock. There was considerable variability among the six strains in ability to cause disease. One of the strains di...

The formation of tumors in tissues of higher plants by the crown-gall organism, (Phytomonas tumefaciens), is influenced by several factors. First, the bacteria themselves must be virulent. Avirulent strains of the crown-gall organism will not produce a gall in susceptible tissue unless that tissue is also stimulated by a growth hormone (1, 10) or by products from a gall produced by fully virule...

Several kinds of primary sunflower (Helianthus annuus) crown gall tissues were established in tissue culture and then labeled in vivo with either [(14)C]arginine, [(14)C]histidine, [(3)H]lysine, or [(3)H]ornithine. Crown gall tissues incited by Agrobacterium tumefaciens strains that utilize octopine as a sole source of carbon or nitrogen for growth synthesized the four members of the N(2)-(1-ca...

cultivar of roses (rosa spp.) has been grown in greenhouses in qazvin region of iran for local markets. agrobacterium tumefaciens strains were isolated and identified from six different samples of roses plants imported from the netherland to iran. during august and september of 2012, nearly 2-5% of rose plants in two different greenhouses in the province of qazvin were observed with crown gall ...

Chromatin was extracted from healthy, avirulent Agrobacterium tumefaciens inoculated, and crown-gall tumor Vicia faba internodes of the same age. Chromatin from crown-gall tissue produced 5 times more RNA per 100 micrograms of DNA than chromatin from the healthy tissue. When template availability was compared using chromatin with saturating amounts of Escherichia coli RNA polymerase, chromatin ...

In the last decade, a number of bacterial species were shown to possess the ability to transmit morphological and biochemical characters to deficient strains. Tumor-inducing ability or virulence in crown-gall bacteria is such a biochemical character, and the acquisition of virulence is here taken to be the acquisition by avirulent strains of bacteria of the ability to synthesize a tumor-inducin...

Tumorigenic strains of Agrobacterium spp. are responsible for crown gall disease of numerous plant species. We present here draft genome sequences of nonpathogenic Agrobacterium nepotum strain 39/7(T) (CFBP 7436(T), LMG 26435(T)), isolated from crown gall tumor on Prunus cerasifera, and tumorigenic Agrobacterium sp. strain KFB 330 (CFBP 8308, LMG 28674), isolated from galls on raspberry.