background: to glean insights into the relationship among hepatitis b virus (hbv) genotype/subgenotypes, a1762t/g1764a mutations and advanced liver disease such as liver cirrhosis (lc) and hepatocellular carcinoma (hcc) in southeast china. methods: a case-control study was performed, consisting of chronic hepatitis b (chb) patients (n=160), lc patients (n=150), and hcc patients (n=156). fluores...

Background: We examine the GA-repeat core promoters of MECOM and GABRA3 in human embryonic kidney-293 cell line and show that those GA-repeats have promoter activity,and those different alleles of the repeats can significantly alter gene expression.We propose a novel role for GA-repeat core promoters to regulate gene expression in the genes involved in development and evolution. Materials and M...

background: hepatitis b virus (hbv) infection is the most common cause of end-stage liver disease in iran. more than 70% of cases are negative for the hepatitis b e antigen (hbeag) and have a long-term response to antiviral therapy, as well as a high relapse rate after discontinuing lamivudine or interferon. these features could be due, in part, to genetic variation of the hbv genome. materials...

â  hepadnaviruses employ an unusual strategy for the production of enormous number of virions during replication which makes rapid and substantial genetic sequence changes and alterations. the pathogenesis and clearance of hepatitis b virus (hbv) infection are engaged by the selection and expression of viral mutants durâ­ing virus-host interactions. mutations in regulatory regions such as the b...

background association of hepatitis b virus (hbv) genotype c with hepatocellular carcinoma (hcc) development remains controversial. hbv basal core promoter (bcp) double mutations (t1762a1764) are very strong confounding factors of genotypes b and c in hcc development. objectives to investigate the association of hbv genotype c with hcc development after controlling for bcp double mutations. mat...

DNA coding for the core antigen from hepatitis B Virus (HBcAg) was amplified, cloned and propagated in E. coli. The core protein was expressed in E. coli and the product was readily detected by Western blot. This protein can be used as a diagnostic material in serum screening tests. To increase the level of expression of this antigen in bacteria, two plasmids were constructed in which the gene ...

background more than two billion people have been exposed to hepatitis b virus (hbv) worldwide. furthermore, four hundred million of them are infected with chronic hbv infection. the predominant mutation of the precore region involves a g to a change at nucleotide1896, which creates a premature stop codon at codon 28. two mutations of a1762t and g1764a are reported as the most prevalent mutatio...

The core promoter, which is generally defined as the region to which RNA Polymerase II is recruited to initiate transcription, plays a pivotal role in the regulation of gene expression. The core promoter consists of different combinations of several short DNA sequences, termed core promoter elements or motifs, which confer specific functional properties to each promoter. Earlier studies that ex...