when grown in monolayer culture at low density and with the addition of serum, cartilage cells dedifferentiate or are overgrown by fibroblast-like cells. the aim of this study was to optimize the cultivation of chondrocytes in monolayer culture and to slow down their transformation or their overgrowth by fibroblast-like cells. the interaction between chondrocytes and cartilage-specifc matrix is...

objective(s) articular cartilage tissue defects cannot be repaired by the proliferation of resident chondrocytes. autologous chondrocyte transplantation (act) is a relatively new therapeutic approach to cover full thickness articular cartilage defects by in vitro grown chondrocytes from the joint of a patient. therefore, we investigated the redifferentiation capability of human chondrocytes mai...

Background: Lead is one of the sustainable metals with devastating effects on many tissues. This study, examined the adverse effect of lead poisoning on the gene expression of collagen type II and osteocalcin by mesenchymal stem cells (MSCs) cultured in chondrogenic and osteogenic media, respectively. Methods: We used 18 male Wistar rats, divided in 3 groups. In addition to libitum feed as the...

objective(s): osteoarthritis is one of the most common diseases in middle-aged population in the world. cartilage tissue engineering (te) has been presented as an effort to introduce the best combination of cells, biomaterial scaffolds and stimulating growth factors to produce a cartilage tissue similar to the natural articular cartilage. in this study, the chondrogenic potential of adipose der...

  Objective(s): Osteoarthritis is one of the most common diseases in middle-aged population in the world. Cartilage tissue engineering (TE) has been presented as an effort to introduce the best combination of cells, biomaterial scaffolds and stimulating growth factors to produce a cartilage tissue similar to the natural articular cartilage. In this study, the chondrogenic potential of adipose d...

background: bone marrow mesenchymal stem cells (mscs) are one of the undifferentiated multipotential cell sources of human body. methods: mscs have the capacity to form a variety of cell types, especially chondrocytes and osteocytes. learning about responses of mscs to external milieu and chemical factors such as ph could recommend new approaches for preparation of suitable scaffolds for bone a...

objective(s): epidermolysis bullosa is one of the most important series of mechano-bullous heritable skin disorders which is categorized into four major types according to the layer that bullae forms within basement membrane zone. in dystrophic form of the disease, blisters are made in the sublamina densa zone, at the level of type vii collagen protein which produce anchoring fibrils. type vii ...

background: micropatterning is becoming a powerful tool for studying cells in vitro. this method not only uses very small amount of material but also mimic the microenvironment structure present in living tissues better than flask culturing techniques. in previous studies using micropatterning of extracellular matrix proteins on glass surfaces, the rate of protein detachment from the surface wa...