نتایج جستجو برای: Co-cultureDifferentiationEmbryonic stem cellIn vitro derived germ cellsSertoli cell

تعداد نتایج: 2661457  

Journal: :iranian journal of basic medical sciences 0
mohammad miryounesi department of medical genetics, school of medicine, tehran university of medical sciences, tehran, iran karim nayernia geneocell, advanced molecular & cellular technologies, montreal, canada mahdi dianatpour department of medical genetics, school of medicine, shiraz university of medical sciences, shiraz, iran fatemeh mansouri department of medical genetics, school of medicine, tehran university of medical sciences, tehran, iran mohammad hossein modarressi* department of medical genetics, school of medicine, tehran university of medical sciences, tehran, iran

objective(s): sertoli cells support in vivo germ cell production; but, its exact mechanism has not been well understood. the present study was designed to analyze the effect of sertoli cells in differentiation of mouse embryonic stem cells (mescs) to germ cells.   materials and methods: a fusion construct composed of a stra8 gene promoter and the coding region of enhanced green fluorescence pro...

Journal: :avicenna journal of medical biotechnology 0

research about potential use of stem cells for the development of germ line cells in vitro had been challenged. in the present study, we reported a novel protocol consisting of cocktail growth factor addition for germ cell differentiation followed by transfection. the cells were purificated based on the expression on the cell surface of a protein. this protein is not present in normal cells of ...

Journal: :international journal of reproductive biomedicine 0
mohammad mehdi akhondi arash mohazzab mahmood jeddi-tehrani mohammad reza sadeghi akram eidi abbas khodadadi

background: spermatogonial stem cells (sscs), a subset of undifferentiated type a spermatogonia, are the foundation of complex process of spermatogenesis and could be propagated in vitro culture conditions for long time for germ cell transplantation and fertility preservation. objective: the aim of this study was in vitro propagation of human spermatogonial stem cells (sscs) and improvement of ...

Fereshte Aliakbari Fereydoon Sargolzaei Aval Hamid Reza Asgari Jafar Ai, Kehinde Adebayo Babatunde Mehdi Abbasi, Mohammad Akbari, Mohammad Taghi Joghataei, Morteza Korouji,

Objective(s): The present day challenge is how to obtain germ cells from stem cells to treat patients with cancer and infertility. Much more efforts have been made to develop a procedure for attaining germ cells in vitro. Recently, human umbilical cord-derived mesenchymal stem cells (HUMSCs) have been introduced with higher efficacy for differentiation. In this work, we tried to explore the eff...

Journal: :international journal of reproductive biomedicine 0
karim nayernia jae ho lee wolfgang engel jessica nolte nadja drusenheimer kristina rathsack

germline and somatic stem cells are distinct types of stem cells that are dedicated to reproduction and somatic tissue regeneration, respectively. germline stem cells (gscs), which can self-renew and generate gametes, are unique stem cells in that they are solely dedicated to transmit genetic information from generation to generation. we developed a strategy for the establishment of germline st...

Journal: :international journal of reproductive biomedicine 0
maryam hosseinzadeh shirzeily parichehr pasbakhsh fardin amidi kobra mehrannia

background: recent publications about differentiation of stem cells to germ cells have motivated researchers to make new approaches to infertility. in vitro production of germ cells improves understanding differentiation process of male and female germ cells. due to the problem of using embryonic stem cells (esc), it’s necessary the mentioned cells be replaced with some adult multi-potent stem ...

Journal: :iranian journal of basic medical sciences 0
hamid reza asgari department of anatomy, faculty of medicine, tehran university of medical sciences, tehran, iran mohammad akbari department of anatomy, faculty of medicine, tehran university of medical sciences, tehran, iran mehdi abbasi department of anatomy, faculty of medicine, tehran university of medical sciences, tehran, iran jafar ai department of tissue engineering, school of advanced technologies, tehran university of medical sciences, tehran, iran morteza korouji department of anatomy, faculty of medicine, iran university of medical sciences, tehran, iran fereshte aliakbari department of anatomy, faculty of medicine, tehran university of medical sciences, tehran, iran

objective(s): the present day challenge is how to obtain germ cells from stem cells to treat patients with cancer and infertility. much more efforts have been made to develop a procedure for attaining germ cells in vitro. recently, human umbilical cord-derived mesenchymal stem cells (humscs) have been introduced with higher efficacy for differentiation. in this work, we tried to explore the eff...

Gentile L H Ko K Sabour D, Sch Stehling M

The germ cell nuclear factor (GCNF) is a member of the nuclear receptor super family of transcription factors. GCNF expression during gastrulation and neurulation is critical for normal embryogenesis in mice. GCNF represses expression of the POU domain transcription factor Oct4 during mouse post-implantation development in vivo. Oct4 is thus down-regulated during female gonadal development, whe...

Mansoureh Movahedin, Mehdi Forouzandeh-Moghadam Zohreh Makoolati

Background Specific growth factors and feeder layers seem to have important roles in in vitro embryonic stem cells (ESCs) differentiation. In this study,the effects of bone morphogenetic protein 4 (BMP4) and mouse embryonic fibroblasts (MEFs) co-culture system on germ cell differentiation from mouse ESCs were studied. MaterialsAndMethods Cell suspension was prepared from one-day-old embryoid bo...

Journal: :iranian journal of basic medical sciences 0
nahid ataie nejad department of reproductive biology, faculty of medicine, tehran university of medical sciences, tehran, iran fardin amidi department of anatomical sciences, faculty of medicine, tehran university of medical sciences, tehran, iran marziyeh agha hoseini ivf center, shariati hospital, tehran university of medical sciences, tehran, iran karim nayer nia institute for molecular medicine and cell therapy, düsseldorf, germany and geneocell, advanced molecular & cellular technologies, tehran, iran mehryar habibi blood transfusion research center, high institute for research and education in transfusion medicine, tehran, iran abdol mohammad kajbafzadeh pediatric urology research center, children hospital medical center, tehran university of medical sciences, tehran, iran

objective(s):mesenchymal stem cells (mscs) derived from wharton’s jelly (wj-mscs) are now much more appealing for cell-based infertility therapy. hence, wj-mscs differentiation toward germ layer cells for cell therapy purposes is currently under intensive study. materials and methods: mscs were isolated from human wharton’s jelly and treated with bmp4, retinoic acid (ra) or co-cultured on human...

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