A high-performance liquid chromatographic method was developed for the analysis of capsaicinoid compounds, the pungent principles of capsicum fruits. A sequential simplex method was applied to optimize the chromatographic response function used to assess the quality of separation by varying the chromatographic parameters. The separation was achieved in 11 min using a C-8 column of 15-cm length ...

A high-performance liquid chromatographic method for the separation of nucleotide glycation reaction product(s) has been developed. The product(s) arising during in vitro glycation of a nucleotide (AMP, GMP or CMP) with a reducing sugar (ribose or glucose) were clearly resolved from the non-glycated constituents of the reaction mixture. The peak(s) of the glycated product(s) increased when an a...

Reduction of folic acid to the tetrahydro level introduces a second asymmetrical center into the molecule at carbon atom 6.1 The resulting tetrahydrofolate, and subsequent derivatives, prepared chemically consist of dl mixtures that are only 50% active in biological reactions. The need for fully reactive isomers of reduced folates led to the enzymatic synthesis of tetrahydrofolate from 7, %dihy...

Chromatographic procedures were developed and successfully employed for the separation of a high number of organic and inorganic compounds present at trace level in complicated accompanying matrices. The capacity of chromatographic separation technologies can be increased by the modification of both the stationary and the mobile phase of the system. Because of their specific adsorption characte...