Fusarium head blight (FHB) caused by Fusarium graminearum is responsible for billions of dollars in agriculture losses. The goal of the present study was evaluation the expression of acidic chitinase, one of PR proteins, in wheat defense response against different FHB induced treatments in 'Falat' as a highly susceptible and 'Sumai3' as a tolerant cultivar. These treatments contained fungi extr...

conclusions in conclusion, chit1 24 bp duplication might not be a candidate gene for susceptibility to ptb. larger studies are necessary to confirm these findings in various populations. results homozygous wild type, heterozygous and homozygous mutant frequencies of chit1 24 bp duplication polymorphism were 43.9%, 43.9% and 12.2% in controls and 42.8%, 45.1% and 12.1% in ptb patients. we found ...

fusarium head blight (fhb) caused by fusarium graminearum is responsible for billions of dollars in agriculture losses. the goal of the present study was evaluation the expression of acidic chitinase, one of pr proteins, in wheat defense response against different fhb induced treatments in 'falat' as a highly susceptible and 'sumai3' as a tolerant cultivar. these treatments contained fungi extr...

cotton cultivar coker has been already transformed with recombinant pbi121-chi via agrobacterium tumefaciens. the t-dna region of pbi121-chi carries the chitinase (chi ) gene from bean and is under the control of the camv35s promoter. t1 and t2 progenies of transgenic cotton containing the chi gene were used in this study.  polymerase chain reaction (pcr), southern and western blotting data con...

ABSTRACT: Streptomyces sampsonii is a kind of biocontrol bacterium with antifungal effects, and chitinase one the main substances. To improve further study structure function gene S. sampsonii, we amplified target fragment by PCR, ligated to expression vector pET-32a, introduced resulting plasmid into Escherichia coli BL21 (DE3) induced chitinase. Then, recombinant was purified is-labelled prot...

Potato (Solanum tuberosum L.) an agro-economically important food crop in the world, is sensitive to many fungal pathogens including Rhizoctonia solani (AG-3), the causal agent of stem and root rot diseases. Chitinase and glucanase are cell wall degrading enzymes which have been shown to have high antifungal activity against a wide range of phytopathogenic fungi. In the present study, plasmid p...

Chitinases are hydrolytic enzymes that have been employed in biotechnology in attempts to increase plants' resistance against fungal pathogens. Genetically modified plants have given rise to concerns of the spreading of transgenes into the environment through vertical or horizontal gene transfer (HGT). In this study, chitinase-like sequences from silver birch (Betula pendula) EST-libraries were...

Chitinase plays a positive role in the pathogenicity of Bacillus thuringiensis to insect pests. We used touchdown PCR to clone the chitinase (Schi) gene from Bacillus thuringiensis serovar sotto (Bt sotto) chromosomal DNA. Our DNA sequencing analysis revealed that the Bt sotto Schi gene consists of an open reading frame (ORF) of 2067 nucleotides with codes for the chitinase precursor. We also f...

A gene encoding chitinase from Serratia marcescens BJL200 was cloned into a broad-host-range vector (pRK415) and mobilized into Sinorhizobium fredii USDA191. Chitinolytic activity was detected in S. fredii USDA191 transconjugants that carried the S. marcescens chiB gene. Chitinase-producing S. fredii USDA191 formed nodules on soybean cultivar McCall. However, there was a delay in nodule formati...

A sediment core spanning approximately 1,600 years was collected from a lake on Ardley Island, Antarctica. The sediment core had been greatly influenced by penguin guano. Using molecular methods, the chitinolytic bacterial community along the sediment core was studied over its entire length. Primers targeting conserved sequences of the catalytic domains of family 18 subgroup A chitinases detect...