background: in this feasibility study, a mechanically loaded in-vitro tissue culture model of joint morphogenesis using the isolated lower extremity of the 8 day old chick embryo was developed to assess the effects of mechanical loading on joint morphogenesis. methods: the developed in-vitro system allows controlled flexion and extension of the chick embryonic knee with a range of motion of 20 ...

This study aimed to investigate the anti-angiogenic activity of Vitex agnus castus methanol extract in vivo. Eggs were incubated for three days, small whole made on the fine pinpoint, next day the egg’s sac penetrated and a small frame was made in the shell. The window was resealed and eggs were returned to the incubator until day 10 of chick embryo development, 20 µl of 500mg/ml of the methano...

this study aimed to investigate the anti-angiogenic activity of vitex agnus castus methanol extract in vivo. eggs were incubated for three days, small whole made on the fine pinpoint, next day the egg’s sac penetrated and a small frame was made in the shell. the window was resealed and eggs were returned to the incubator until day 10 of chick embryo development, 20 µl of 500mg/ml of the methano...

 Background & Objective:  Toxico-pathological effects of herbal plants have always been a major concern, but little information is provided on the toxico-pathological effects of origanum on the fetus. Due to ethical rules and regulations, no drug experiment on the human fetus is permitted; hence, the chick embryo model is used as an ideal opportunity to study the adverse effects of drugs. This ...

Chick embryo extract is widely used to induce differentiation in cultures of muscle and other tissues. As part of a systematic study of factors which promote maximal differentiation of muscle cells, we found that unmodified chick embryo extract could be quite toxic to cells. This report describes the effects of varying culture media composition and cell density on the rate and extent of differe...

Chick embryo heart cells were used in coverslip cultures. The medium consisted of: Chick embryo extract 15% Phenol red solution (.04%) 7% Tyrode solution 28% Chicken plasma 50% Cultures were incubated for 72 hours at 37°C. The amount of inhibition of growth was determined from comparisons of the areas of growth and of the mitotic indexes. Many of the compounds were synthesized in this Laborato...

Change in sex ratios for broiler chickens, has many economic benefits of the poultry industry. The gonads in chick embryo at an early stage have the potential to convert to both sexes (male and female). Sexual differentiation is due to aromatase expression in the left gonad in 5-6 days of the embryonic period and the production of estrogen from testosterone. Administration of aromatase inhibito...

The study aimed to investigate the effect of in ovo (IO) leptin injection on yolk sac concentration, embryo development, blood lipid metabolism, and biochemical parameters broiler chicks during early post-hatching period. Hatching eggs were weighed placed an incubator with 100 eggs/4 replications/group. groups were: non-injected group (C); 3 injected μl phosphate buffer solution (PBS); 0.5 (L0....

Aqueous extracts of 11-day-old chick embryos enhance the growth of tubercle bacilli in oleic acid-albumin media. These extracts also increase the tendency of virulent strains to exhibit the serpentine pattern of growth and confer this property on avirulent variants which normally grow unoriented in clumps. Growth in the presence of chick embryo extract slightly increases the virulence of the vi...

the purpose of this experimental study was to evaluate the effect of chicken amniotic fluid (af) on a cross section of rat sciatic nerves. thirty adult male sprague-dawley rats weighing 275 to 300 g, were randomized into three groups treated with (1) amniotic fluid or af (n=10), (2) normal saline or ns (n=10), and (3) sham surgery (n=10). the af was aspirated from the amniotic cavity of incubat...