The School of Biological Sciences, 1.800 Stopford Building, University of Manchester, Manchester M13 9PT, UK Fusarium gramhearum was grown in batch and continuous (chemostat) culture on a glucose-mineral salts medium in the presence and absence of casein. In the absence of casein no protease activity was detected in the culture filtrate from either batch or chemostat culture. For batch cultures...

The influence of dilution rate on the production of biomass, ethanol, and invertase in an aerobic culture of Succharornyces carlsbergensis was studied in a glucoselimited chemostat culture. A kinetic model was developed to analyze the biphasic growth of yeast on both the glucose remaining and the ethanol produced in the culture. The model assumes a double effect where glucose regulates the flux...

In synchronized continuous cultures of Saccharomyces cerevisiae CBS 8066, the production of the extracellular invertase (EC 3.2.1.26) showed a cyclic behavior that coincided with the budding cycle. The invertase activity increased during bud development and ceased at bud maturation and cell scission. The cyclic changes in invertase production resulted in cyclic changes in amounts of invertase l...

The aim of this course is to describe the mathematical model of competition in the chemostat and to study various mechanisms of coexistence of species. Basically, the chemostat consists of a nutrient input, pumped at a constant rate into a well-mixed culture vessel. The culture vessel contains the microorganisms that are growing and competing for the nutrient. Volume is kept constant by pumping...

Continuous culture of Bacillus popilliae was achieved for the first time in a small chemostat. Initially, variable cell yields during steady-state chemostat growth led to a re-examination of growth rates in batch cultures. B. popilliae NRRL B-2309 and a wild strain were both found to be natural mixtures of three substrains characterized by different growth rates and colony morphologies and vary...

The use of chemostat culture facilitates the careful comparison of different yeast strains growing in well-defined conditions. Variations in physiology can be measured by examining gene expression, metabolite levels, protein content, and cell morphology. In this protocol, we show how a combination of sample types can be collected during harvest from a single 20-mL chemostat in a ministat array,...

two different growth media, namely complex and defined media, were used to examine establishment of steady-state conditions in phosphate-limited culture system of saccharomyces cerevisiae cen.pk113-5d strain. using the defined growth medium, it was possible to obtain steady state condition in the continuous culture. the effect of phosphate concentration on the growth of s. cerevisiae in phospha...

1 Review of competition for a single substrate Paul Waltman’s chemostat-related work has had a large impact in population biology, ecology and bio-engineering. It has motivated and inspired the work of many other authors, including us. It seems appropriate to point out a major open problem which remains unresolved after more than thirty years and to touch on some other issues related to bacteri...

The interaction between Escherichia coli O157:H7 and its specific bacteriophage PP01 was investigated in chemostat continuous culture. Following the addition of bacteriophage PP01, E. coli O157:H7 cell lysis was observed by over 4 orders of magnitude at a dilution rate of 0.876 h(-1) and by 3 orders of magnitude at a lower dilution rate (0.327 h(-1)). However, the appearance of a series of phag...