homogenizer and ultrasonicator that we call as mechanical methods were used in the lab scale production of alcohol free reduced glutathione (alcohol free gsh) from baker’s yeast. the study was done to determine the effects of specific parameters of the methods and the ph of the medium on the production of the gsh. the cell disruptions were run at a wide range of those parameters at the suitable...

Background: Despite several studies many questions remain regarding how varicocele develops its adverse effect on testicular tissue. Spermatogenesis cell lineage needs carbohydrates as a main source of energy for cell division. Any disruption in glucose transporting system in seminiferous tubules results in remarkable decrease in germinal cells biological function. Therefore present study was c...

Traumatic brain injury (TBI) is a disruption in the brain functions following a head trauma. Cell therapy may provide a promising treatment for TBI. Human neural stem cells cultured in self-assembling peptide scaffolds have been proposed as a potential novel method for cell replacement treatment after TBI. In the present study, we accessed the effects of human neural stem/progenitor cells (hNS/...

background: the sertoli cells in the testis create unique and safe environment to protect seminiferous tubules from auto antigens and invading pathogens. these cells produce the survival factor of the blood-testis barrier and produce special materials such as androgen binding proteins and contribute to the coordinated action of spermatogenesis. given that the sertoli cells play an essential rol...

Cadmium (Cd) is one of the most toxic environmental heavy metals to organisms. The prominent toxic effects of Cd on reproductive organs are very well known. Gonad histopathology is a valuable tool for the assessment of endocrine disruption compounds EDCs effects on fish. The aim of this study was to evaluate the reproductive toxicity of cadmium chloride (CdCl2) on histopathology of Zebrafish (D...

there are many techniques to knock out directed genes in bacteria, some of which have been described in salmonella species. in this study, a combination of soeing pcr method and the λ red disruption system were used to disrupt phop gene in wild type and standard strains of salmonella typhimurium. three standards pcr and one fusion pcr reactions were performed to construct a linear dna including...

a neurological disorder is any disorder of the nervous system is usually due to cell loss. the ultimate goal of cns therapy is to replace lost cells and tissue. there is no neuroprotective, pharmacological and effective treatment available that restores the injury-induced loss of function. recent studies suggest that identification of adult neural stem/progenitor cells indwelling in the neuroge...