purpose: to describe a novel surgical technique for in vivo limbal stem cell cultivation for reconstruction of corneal epithelium in total limbal stem cell deficiency (lscd). methods: patients suffering from chronic total unilateral lscd due to a chemical injury were sequentially included. one small limbal block (2x1 mm) harvested from contralateral healthy eye sutured over the amniotic membran...

application of nanotechnology in medicinal and biological fields has attracted a great interest in the recent yeras. in this paper the cell membrane leakage induced by iron nanoparticles (fe-np) against pc12 cell line which is known as a model of nervous system cell line was investigated by the lactate dehydrogenase (ldh) test. therefore, pc12 cells were incubated with different concentration o...

sulfonated poly (ether ether ketone) (speek) with a low degree of sulfonation (ds = 40%) was prepared for proton exchange membrane fuel cells (pemfc). poly (ether ether ketone) (peek) was sulfonated in concentrated h2so4 under n2 atmosphere and characterized by the hydrogen nuclear magnetic resonance (h-nmr) technique. after preparation of the speek polymer, the obtained polymer was dissolved i...

background and objective: basal cell carcinoma (bcc) and squamous cell carcinoma (scc) are two common tumors of the skin. in some cases, distinction between bcc and scc can be difficult. this study aimed to clarify this uncertainty through immunohistochemical analysis. in this respect, epithelial membrane antigen (ema) and ber-ep4 are the two immunohistochemical markers on which we focus in dif...

nowadays, growing interest in the application of nanotechnology in biomedical and biotechnological fields has opened a new avenue to explore the nanoparticles-biological system interaction. indeed, a clear gap is still in the cytotoxic effect of nps on the biological systems. for this purpose, the interaction of the silica oxide nanoparticles (sio2-np) with pc12 cell line, as a model of nervous...

background: nowadays, highly specific aptamers generated by cell selex technology (systematic evolution of ligands by exponential enrichment) are being applied for early detection of cancer cells. prostate specific membrane antigen (psma), over expressed in prostate cancer, is a highly specific marker and therefore can be used for diagnosis of the prostate cancer cells. the aim of the present s...

in present research work, homogeneous anion exchange membranes based on polysulfone (qapsfs) were prepared via chloromethylation, amination and alkalization. in amination step, trimethylamine and n,n,n',n'-tetramethyl-1,6-hexanediamine were used as amination and crosslinking agents, respectively. the chloromethylated polysulfone was characterized by 1hnmr spectroscopy and chloromethylation degr...

mitochondrial functions have a major impact on t-cell functionality. in this study we characterized whether mitochondrial function in the neonatal t-cells differs from that in the adult t-cells during short t-cell activation. methods: we used fow cytometry methods to test mitochondrial mass and to monitor mitochondrial ca2+ levels, mitochondrial potential and superoxide generation in parallel w...

This communication demonstrates the relevance of membrane sealing in a test cell to its performance. Membranes need to be sealed, and therefore a more or less signifcant (depending on the test cell design) peripheral part of the membrane is supported directly by the cell body (instead of a permeate spacer). Although it may seem that there should be no fltration through ...

purpose: to report the early results of transplantation of autologous limbal stem cell cultivated on amniotic membrane in total limbal stem cell deficiency. methods: four eye of 4 patients with total unilateral stem cell deficiency secondary to severe chemical or thermal burn were included. stem cell deficiency was confirmed with impression cytology in all cases. under topical anesthesia, a sma...