objective(s): renal cell carcinoma (rcc) is insensitive to conventional chemotherapy. ginkgetin effectively treats several carcinoma cells. however, little is known about effects of ginkgetin on rcc. in the present study, using 786-o cells, we evaluate whether ginkgetin exerts anticancer effects against rcc. materials and methods: 786-o cells suspended in the medium containing ginkgetin were cu...

Houttuynia cordata Thunb. is an edible and medicinal plant that belongs to the family of Saururaceae widely distributes in Eastern Asia countries. H. have been reported as a promising anticancer agent against various cancer cells. This study was determine anti-proliferative effect extract on gastric cells using vitro experimental model. The cytotoxicity examined via 2,5-diphenyl-2H-tetrazolium ...

introduction: repetitive cortical spreading depression (csd) can lead to cell death in immature brain tissue. caspases are involved in neuronal cell death in several csd-related neurological disorders. yet, whether repetitive csd itself can induce caspase activation in adult or juvenile tissue remains unknown. inducing repetitive csd in somatosensory cortices of juvenile and adult rats in vivo,...

the biological application of nanoparticles (nps) is a rapidly developing area of nanotechnology that raises new possibilities in the treatment of human cancers. the cytotoxicity was evaluated by mtt and ldh assays. the apoptotic effect of free icd-85 and icd-85 nps on hela cells was assessed using caspase-8 colorimetric assay. the mtt assay showed that icd-85 nps could enhance the in-vitro cyt...

objective(s): juglone is isolated from many species of the juglandaceae family and used as an anti-viral, anti-bacterial, and anti-tumor therapeutic. here, we evaluated juglone-induced antitumor effect in ovarian cancer skov3 cells. materials and methods: mtt assay was performed to examine juglone anti-proliferative effect. cell cycle and apoptosis were studied using flow cytometry in juglone-tr...

cladribine, an analogue of deoxyadenosine, is highly toxic for both non-dividing and proliferating cells and has shown activity in the treatment of several malignancies. therefore, the aim of the present study is to investigate the cytotoxicity effect of cladribine (2-cda) on the breast cancer cell line, mcf-7 (estrogen receptor positive, er+). mtt assay, annexin v-fluorescein/pi and hoechst 33...

Introduction: Repetitive cortical spreading depression (CSD) can lead to cell death in immature brain tissue. Caspases are involved in neuronal cell death in several CSD-related neurological disorders. Yet, whether repetitive CSD itself can induce caspase activation in adult or juvenile tissue remains unknown. Inducing repetitive CSD in somatosensory cortices of juvenile and adult rats in vivo,...

objective: this study aimed to evaluate the incidence of apoptosis in vitrified and non-vitrified human ovarian tissue by the use of morphological analysis and apoptosis assay techniques. methods: we obtained human ovarian tissue biopsies from 30 women who underwent elective caesarean sections. tissues were transported to the laboratory in pre-warmed, equilibrated leibovitz l-15 medium within 2...

Background/Aim: We investigated the cytotoxic effects of plumbagin on metastatic retinoblastoma, using highly cell line Y79. Materials and Methods: Effect growth was assessed with water-soluble tetrazolium 1 (WST-1) proliferation assay automated hemocytometry trypan blue-exclusion assay. Cell death studied acridine orange/ethidium bromide live-dead annexin-V-fluorescein isothiocyanate/propidium...

Background: Artesunate has recently been used in some pharmacological preparation to induce tumor cell apoptosis. The drug is a semi-synthetic derivative of artemisinin, traditionally used for its antimalarial. However, up to now, its anticancer mechanism against diff erent types of tumors is not known.Objectives: The most important purposes of the present research was fi...