Tenderness is the single most important factor influencing consumer acceptance of meat. The calpain proteolytic system is known to be responsible for the post-mortem tenderization of meat. The purpose of this study was to determine and compare the tensile strength and total calpain activities in different muscles of camel, cattle, sheep and goat. In camels, the effect of age and sex of animal w...

tenderness is the single most important factor influencing consumer acceptance of meat. the calpain proteolytic system is known to be responsible for the post-mortem tenderization of meat. the purpose of this study was to determine and compare the tensile strength and total calpain activities in different muscles of camel, cattle, sheep and goat. in camels, the effect of age and sex of animal w...

apoptosis, a form of programmed cell death that occurs under physiological as well as pathological conditions, is characterized by morphological and biochemical features. while the importance of caspases in apoptosis is established, several noncaspase proteases (ca2+-dependent proteases) such as calpain may play a role in the execution of apoptosis. the calpain family consists of two major isof...

There is an urgent need for treatments sporadic Alzheimer’s Disease (sAD). Although antibodies removing ß-amyloid have recently been shown to slow disease progression, the degenerative course continues. Thus, there a strategies that intervene early in process, before irreversible damage done neurons (e.g., by autophagic degeneration). This review will summarize evidence indicating targeting cal...

Tauopathies are neurodegenerative diseases characterized by abnormal metabolism of misfolded tau proteins and progressive. Pathological phosphorylation occurs in the retinal ganglion cells (RGCs) after optic nerve injuries. Cyclin-dependent kinase-5 (Cdk5) causes hyperphosphorylation tau. To determine roles played Cdk5 degeneration, roscovitine, a inhibitor, was injected intravitreally crush (O...

The aim of this study was to investigate the role Ca2+ in process calpain-2 becoming associated myofibrils and potential myofibril-bound calpain degrade myofibrillar proteins. Different concentrations were applied mixed with partially purified calpain-2. induced binding a concentration-dependent manner. half-maximal requirements for proteolysis 0.60 mM 0.29 mM, respectively. To proteolytic acti...

Calpain, a Ca2+-dependent cysteine protease, plays significant role in gene expression, signal transduction, and apoptosis. Mutations human calpain-5 cause autosomal dominant neovascular inflammatory vitreoretinopathy the inhibition of activity may constitute an effective therapeutic strategy for this condition. Although is ubiquitously expressed mammalian tissues was recently found to be prese...

Introduction and Aim: It has been proposed that µ-calpain is responsible for neuronal survival, while m-calpain – the degeneration. can be assumed "susceptibility" to damage factor neurons in different CNS regions depends on content/activity of calpain isoforms. We analyzed mRNA levels activity µ-and structures rats. Materials Methods: After decapitation intact male Wistar rats prefrontal corte...

Systemic injection of kainate produces repetitive seizure activity in both rats and mice. It also results in short-term synaptic modifications as well as delayed neurodegeneration. The signaling cascades involved in both short-term and delayed responses are not clearly defined. The calcium-dependent protease calpain is activated in various brain structures following systemic kainate injection, ...

The objectives of this study were to determine the extent to which pH and ionic strength influence mu- and m-calpain activity and the inhibition of calpains by calpastatin. Calpastatin, mu-calpain, and m-calpain were purified from at-death porcine semimembranosus. Mu-calpain or m-calpain (0.45 U) were incubated with the calpain substrate Suc-Leu-Leu-Val-Tyr-7-amino-4-methyl coumarin in the pres...