The present work describes the effects of winter and spring genotypes of rapeseed (PF7045/91, Okapi and Opera), explants (cotyledons and hypocotyls), light, different kinds and concentrations of auxins [Naphthylacetic acid (NAA) and 2, 4dichlorophenoxyacetic acid (2, 4-D)], presence of cytokinin [benzyladenine (BA)] and different concentrations of micro and macro elements (MS and 1/2MS) on call...

This study provides a physiological analysis of somatic embryogenesis in four elite cultivars of date palms: Ahmar, Amsekhsi, Tijib, and Amaside, from the initial callogenesis to establishment and proliferation of embryogenic suspension cultures. Somatic embryos development and in vitro plants rooting were also studied. For each step, auxins and cytokinins concentrations were optimised. The pri...

The present work aimed at regenerating plants of Eucalyptus camaldulensis from the cotyledonary explants and describing the anatomy of the tissues during callogenesis and organogenesis processes, in order to determine the origin of the buds. The cotyledonary leaves of E. camaldulensis were cultured in Murashige and Skoog (MS), WPM and JADS media supplemented with 2.7 μM NAA and 4.44 μM BAP. The...

In this study, the effect of plant growth regulators on callogenesis and gametic embryogenesis from anther cultures of cucumber (Cucumis sativus L.) was investigated. A factorial experiment based on Completely Randomized Design with three replications was used in this study. Two cultivars of cucumber (Beta Alfa and Esfahani) were used as first factor and 11 hormone treatments including: control...

In this study, we have investigated the effect of copper oxide nanoparticles (CuO-NPs) on callogenesis and regeneration of Oryza sativa L (Super Basmati, Basmati 2000, Basmati 370, and Basmati 385). In this regard, CuO-NPs have been bio-synthesized via Azadirachta indica leaf extract. Scanning electron microscope (SEM) analysis depicts average particle size of 40 ± 5 nm with highly homogenous a...

Leaves from in vitro proliferating shoots of cvs. Chandler and Hartley of Persian walnut were cultured on MS and NN media supplemented with BA and NAA. Three light conditions including darkness, low light intensity and modified light intensity were used. Most of the calli were friable and non-embryonic in all three conditions. Rate of callogenesis depended on cultivar, culture medium, light con...

Male florets and pistillate flowers of cvs.Chandler and Hartley of Persian walnut were cultured on modified Murashige and Skoog medium (MS) supplemented with 0.05 mg l-1 biotin, 0.5 mg l-1 folic acid, 100 mg l-1 glutamine and different concentrations of Naphthalene acetic acid (NAA) and Kinetin (KIN) and for pistillate flowers culture, these concentrations were 0.25, 0.5, 1 and 2 mg l-1). After...

Micropropagation techniques have been widely applied in globe artichoke (C. cardunculus L. var. scolymus), however, efficient protocols for the establishment of in vitro callogenesis and organogenesis, a pre-requisite for Agrobacteriummediated genetic transformation, have not been set up so far. We developed an efficient protocol for callus induction from leaf explants of three globe artichoke ...

A protocol was developed for callus induction and regeneration in tomato (Lycopersicon esculentum) var. Moneymaker. Hypocotyl and leaf disc explants of tomato were used as a starting material for callus induction. Explants were cultured on MS medium having different concentrations of hormones. Maximum callogenesis from hypocotyls was obtained on MS medium supplemented with IAA (2 mg/l), NAA (2 ...

Background and objectives: Hawthorn as an ethno-pharmaceutical plant has been consumed for 2,500 years. Flowers, leaves and fruits of hawthorn are rich in antioxidant and polyphenols and are popular in the treatment of congestive heart failure. Methods: In the present study, by using various media and plant hormones the regeneration and callogenesis capacity of...